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Red shift in the spectrum of a chlorophyll species is essential for the drought-induced dissipation of excess light energy in a poikilohydric moss, Bryum argenteum

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Abstract

Some mosses are extremely tolerant of drought stress. Their high drought tolerance relies on their ability to effectively dissipate absorbed light energy to heat under dry conditions. The energy dissipation mechanism in a drought-tolerant moss, Bryum argenteum, has been investigated using low-temperature picosecond time-resolved fluorescence spectroscopy. The results are compared between moss thalli samples harvested in Antarctica and in Japan. Both samples show almost the same quenching properties, suggesting an identical drought tolerance mechanism for the same species with two completely different habitats. A global target analysis was applied to a large set of data on the fluorescence-quenching dynamics for the 430-nm (chlorophyll-a selective) and 460-nm (chlorophyll-b and carotenoid selective) excitations in the temperature region from 5 to 77 K. This analysis strongly suggested that the quencher is formed in the major peripheral antenna of photosystem II, whose emission spectrum is significantly broadened and red-shifted in its quenched form. Two emission components at around 717 and 725 nm were assigned to photosystem I (PS I). The former component at around 717 nm is mildly quenched and probably bound to the PS I core complex, while the latter at around 725 nm is probably bound to the light-harvesting complex. The dehydration treatment caused a blue shift of the PS I emission peak via reduction of the exciton energy flow to the pigment responsible for the 725 nm band.

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Acknowledgements

We thank Dr. Kojiro Hara (Akita Prefectural University) for kindly advising us on the access to transcriptome data on B. argenteum. This work was supported in part by a Grant-in-Aid for Scientific Research (No. 15H04356 to Y.S. and No. 24770030 to M. K.) from the Japan Society for the Promotion of Science (JSPS).

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  1. Ahmed Mohamed

    Present address: Institut national de la recherche scientifique (INRS-EMT), Varennes, QC, J3X 1S2, Canada

  2. Hiroshi Fukumura

    Present address: National Institute of Technology, 4-16-1 Ayashi-chuo, Aoba-ku, Sendai, 989-3128, Japan

Authors and Affiliations

  1. Department of Chemistry, Graduate School of Science, Tohoku University, Aramaki Aza Aoba, Aoba-Ku, Sendai, 980-8578, Japan

    Yutaka Shibata, Ahmed Mohamed, Koichiro Taniyama, Kentaro Kanatani & Hiroshi Fukumura

  2. Department of Biological Science, Faculty of Science and Engineering, Chuo University, 1-13-27 Kasuga, Bunkyo-Ku, Tokyo, 112-8551, Japan

    Makiko Kosugi

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  1. Yutaka Shibata
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Correspondence to Yutaka Shibata.

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Shibata, Y., Mohamed, A., Taniyama, K. et al. Red shift in the spectrum of a chlorophyll species is essential for the drought-induced dissipation of excess light energy in a poikilohydric moss, Bryum argenteum . Photosynth Res 136, 229–243 (2018). https://doi.org/10.1007/s11120-017-0461-0

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