Photosynthesis Research

, Volume 134, Issue 2, pp 175–182 | Cite as

Chlorophyll a with a farnesyl tail in thermophilic cyanobacteria

  • Jessica M. Wiwczar
  • Amy M. LaFountain
  • Jimin Wang
  • Harry A. Frank
  • Gary W. Brudvig
Original Article

Abstract

Photosystem II (PSII) of oxygenic photosynthetic organisms normally contains exclusively chlorophyll a (Chl a) as its major light-harvesting pigment. Chl a canonically consists of the chlorin headgroup with a 20-carbon, 4-isoprene unit, phytyl tail. We have examined the 1.9 Å crystal structure of PSII from thermophilic cyanobacteria reported by Shen and coworkers in 2012 (PDB accession of 3ARC/3WU2). A newly refined electron density map from this structure, presented here, reveals that some assignments of the cofactors may be different from those modeled in the 3ARC/3WU2 structure, including a specific Chl a that appears to have a truncated tail by one isoprene unit. We provide experimental evidence using high-performance liquid chromatography and mass spectrometry for a small population of Chl a esterified to a 15-carbon farnesyl tail in PSII of thermophilic cyanobacteria.

Keywords

Chlorophyll a CP43 Photosystem II Thermosynechococcus elongatus X-ray crystal structure 

Supplementary material

11120_2017_425_MOESM1_ESM.pdf (239 kb)
Supplementary material 1 (PDF 238 KB)

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  1. 1.Department of Molecular Biophysics and BiochemistryYale UniversityNew HavenUSA
  2. 2.Department of ChemistryUniversity of ConnecticutStorrsUSA
  3. 3.Department of ChemistryYale UniversityNew HavenUSA

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