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Concentration-based self-assembly of phycocyanin

Abstract

Cyanobacteria light-harvesting complexes can change their structure to cope with fluctuating environmental conditions. Studying in vivo structural changes is difficult owing to complexities imposed by the cellular environment. Mimicking this system in vitro is challenging, as well. The in vivo system is highly concentrated, and handling similar in vitro concentrated samples optically is difficult because of high absorption. In this research, we mapped the cyanobacteria antennas self-assembly pathways using highly concentrated solutions of phycocyanin (PC) that mimic the in vivo condition. PC was isolated from the thermophilic cyanobacterium Thermosynechococcus vulcanus and measured by several methods. PC has three oligomeric states: hexamer, trimer, and monomer. We showed that the oligomeric state was changed upon increase of PC solution concentration. This oligomerization mechanism may enable photosynthetic organisms to adapt their light-harvesting system to a wide range of environmental conditions.

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Abbreviations

LHC:

Light-harvesting complexes

PBS:

Phycobilisome

PC:

Phycocyanin

APC:

Allophycocyanin

LS:

Low salt

DW:

Distilled water

DDW:

Double-distilled water

TEM:

Transmission electron microscopy

Cryo-TEM:

Cryogenic transmission electron microscopy

SAXS:

Small-angle X-ray scattering

MS:

Mass spectrometry

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Acknowledgements

This research was supported by a Grant No. 3-12405(151235) from the Ministry of Science, Technology and Space, Israel, the National Science Council (NSC) of Taiwan, the Israel Science Foundation (843/16) and the US-Israel Bi-National Science Fund (2014395).

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Correspondence to Yossi Paltiel.

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Eisenberg, I., Harris, D., Levi-Kalisman, Y. et al. Concentration-based self-assembly of phycocyanin. Photosynth Res 134, 39–49 (2017). https://doi.org/10.1007/s11120-017-0406-7

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  • DOI: https://doi.org/10.1007/s11120-017-0406-7

Keywords

  • Phycocyanin
  • Oligomerization
  • TEM
  • SAXS
  • Native mass spectrometry