Estimation of photosynthesis in cyanobacteria by pulse-amplitude modulation chlorophyll fluorescence: problems and solutions

Review

DOI: 10.1007/s11120-017-0367-x

Cite this article as:
Ogawa, T., Misumi, M. & Sonoike, K. Photosynth Res (2017). doi:10.1007/s11120-017-0367-x
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Abstract

Cyanobacteria are photosynthetic prokaryotes and widely used for photosynthetic research as model organisms. Partly due to their prokaryotic nature, however, estimation of photosynthesis by chlorophyll fluorescence measurements is sometimes problematic in cyanobacteria. For example, plastoquinone pool is reduced in the dark-acclimated samples in many cyanobacterial species so that conventional protocol developed for land plants cannot be directly applied for cyanobacteria. Even for the estimation of the simplest chlorophyll fluorescence parameter, Fv/Fm, some additional protocol such as addition of DCMU or illumination of weak blue light is necessary. In this review, those problems in the measurements of chlorophyll fluorescence in cyanobacteria are introduced, and solutions to those problems are given.

Keywords

Chlorophyll fluorescence Cyanobacteria Nonphotochemical quenching Pulse-amplitude modulation (PAM) fluorometry State transition 

Abbreviations

DCMU

3-(3, 4-Dichlorophenyl)-1, 1-dimethylurea

Fm

Maximum fluorescence determined under oxidized plastoquinone pool conditions

Fm′

Maximum fluorescence under reduced plastoquinone pool conditions

F0

Minimum fluorescence under oxidized plastoquinone pool conditions

F0′

Minimum fluorescence under reduced plastoquinone pool conditions

Fs

Stable fluorescence level

Fv/Fm

Chlorophyll fluorescence parameter indicating the maximum quantum yield of Photosystem II calculated as (Fm − F0)/Fm

NDH

NAD(P)H dehydrogenase

OCP

Orange carotenoid protein

PFD

Photon flux density

PQ

Plastoquinone

PS

Photosystem

Funding information

Funder NameGrant NumberFunding Note
Ministry of Education, Culture, Sports, Science, and Technology
  • 16H06552
  • 16H06553
  • 16H04809
Japan Society for the Promotion of Science
  • 26-7221

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.Faculty of Education and Integrated Arts and SciencesWaseda UniversityTokyoJapan
  2. 2.Japan Society for the Promotion of ScienceTokyoJapan

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