Photosynthetic fuel for heterologous enzymes: the role of electron carrier proteins

  • Silas Busck Mellor
  • Konstantinos Vavitsas
  • Agnieszka Zygadlo Nielsen
  • Poul Erik Jensen
Review

DOI: 10.1007/s11120-017-0364-0

Cite this article as:
Mellor, S.B., Vavitsas, K., Nielsen, A.Z. et al. Photosynth Res (2017). doi:10.1007/s11120-017-0364-0

Abstract

Plants, cyanobacteria, and algae generate a surplus of redox power through photosynthesis, which makes them attractive for biotechnological exploitations. While central metabolism consumes most of the energy, pathways introduced through metabolic engineering can also tap into this source of reducing power. Recent work on the metabolic engineering of photosynthetic organisms has shown that the electron carriers such as ferredoxin and flavodoxin can be used to couple heterologous enzymes to photosynthetic reducing power. Because these proteins have a plethora of interaction partners and rely on electrostatically steered complex formation, they form productive electron transfer complexes with non-native enzymes. A handful of examples demonstrate channeling of photosynthetic electrons to drive the activity of heterologous enzymes, and these focus mainly on hydrogenases and cytochrome P450s. However, competition from native pathways and inefficient electron transfer rates present major obstacles, which limit the productivity of heterologous reactions coupled to photosynthesis. We discuss specific approaches to address these bottlenecks and ensure high productivity of such enzymes in a photosynthetic context.

Keywords

Ferredoxin Flavodoxin Synthetic biology Metabolic engineering Cytochrome P450 Photosynthesis 

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Silas Busck Mellor
    • 1
  • Konstantinos Vavitsas
    • 1
  • Agnieszka Zygadlo Nielsen
    • 1
  • Poul Erik Jensen
    • 1
  1. 1.Copenhagen Plant Science Center, Center for Synthetic Biology ‘bioSYNergy’, Department of Plant and Environmental SciencesUniversity of CopenhagenFrederiksberg CDenmark