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A two-component nonphotochemical fluorescence quenching in eustigmatophyte algae

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Abstract

Eustigmatophyte algae represent an interesting model system for the study of the regulation of the excitation energy flow due to their use of violaxanthin both as a major light-harvesting pigment and as the basis of xanthophyll cycle. Fluorescence induction kinetics was studied in an oleaginous marine alga Nannochloropsis oceanica. Nonphotochemical fluorescence quenching was analyzed in detail with respect to the state of the cellular xanthophyll pool. Two components of nonphotochemical fluorescence quenching (NPQ), both dependent on the presence of zeaxanthin, were clearly resolved, denoted as slow and fast NPQ based on kinetics of their formation. The slow component was shown to be in direct proportion to the amount of zeaxanthin, while the fast NPQ component was transiently induced in the presence of membrane potential on subsecond timescales. The applicability of these observations to other eustigmatophyte species is demonstrated by measurements of other representatives of this algal group, both marine and freshwater.

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Abbreviations

AL:

Actinic illumination

Ant:

Antheraxanthin

Chl:

Chlorophyll

DCMU:

3-(3,4-Dichlorophenyl)-1,1-dimethylurea

DEPS:

Xanthophyll pool de-epoxidation state

NPQ:

Nonphotochemical (Chl a fluorescence) quenching

PSII:

Photosystem II

SP:

Saturating pulse

Vio:

Violaxanthin

Zea:

Zeaxanthin

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Acknowledgments

This work was supported by the Czech Science Foundation grant 14-01377P and by institutional funding RVO:60077344. K.B. was involved in the present work during his participation in a secondary school student scientific project competition. The authors would like to thank Vladimír Špunda for fruitful discussion on the ‘low-wave’ phenomenon. Expert technical assistance of Mr. František Matoušek is gratefully acknowledged.

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Authors and Affiliations

  1. Institute of Plant Molecular Biology, Biology Centre CAS, Branišovská 31, 370 05, České Budějovice, Czech Republic

    David Bína, Karel Bouda & Radek Litvín

  2. Faculty of Science, University of South Bohemia, Branišovská 1760, 370 05, České Budějovice, Czech Republic

    David Bína, Karel Bouda & Radek Litvín

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  1. David Bína
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Correspondence to Radek Litvín.

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Bína, D., Bouda, K. & Litvín, R. A two-component nonphotochemical fluorescence quenching in eustigmatophyte algae. Photosynth Res 131, 65–77 (2017). https://doi.org/10.1007/s11120-016-0299-x

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