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Synechocystis mutants defective in manganese uptake regulatory system, ManSR, are hypersensitive to strong light

Abstract

High affinity transport of manganese ions (Mn2+) in cyanobacteria is carried by an ABC-type transporter, encoded by the mntCAB operon, which is derepressed by the deficiency of Mn2+. Transcription of this operon is negatively regulated by the two-component system consisting of a sensory histidine kinase ManS and DNA-binding response regulator ManR. In this study, we examined two Synechocystis mutants, defective in ManS and ManR. These mutants were unable to grow on high concentrations of manganese. Furthermore, they were sensitive to high light intensity and unable to recover after short-term photoinhibition. Under standard illumination and Mn2+ concentration, mutant cells revealed the elevated levels of transcripts of genes involved in the formation of Photosystem II (psbA, psbD, psbC, pap-operon). This finding suggests that, in mutant cells, the PSII is sensitive to high concentrations of Mn2+ even at relatively low light intensity.

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Abbreviations

Hik:

Histidine kinase

Rre:

Response regulator

ORF:

Open reading frame

PS II:

Photosystem II

RC:

Reaction center

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Acknowledgments

This work was supported by grants from Russian Science Foundation (no. 04-24-00020) to DAL and from Russian Foundation for Basic Research (13-04-01767) to VVZ.

Author information

Correspondence to Dmitry A. Los.

Additional information

Guest Editor: Suleyman Allakhverdiev.

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Zorina, A., Sinetova, M.A., Kupriyanova, E.V. et al. Synechocystis mutants defective in manganese uptake regulatory system, ManSR, are hypersensitive to strong light. Photosynth Res 130, 11–17 (2016). https://doi.org/10.1007/s11120-015-0214-x

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Keywords

  • Synechocystis
  • Cyanobacteria
  • Ions
  • Manganese
  • Sensors
  • Stress
  • Transcription factor
  • Transporter