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Effects of ocean acidification on the photosynthetic performance, carbonic anhydrase activity and growth of the giant kelp Macrocystis pyrifera

Photosynthesis Research volume 124, pages 293–304 (2015)Cite this article

Abstract

Under ocean acidification (OA), the 200 % increase in CO2(aq) and the reduction of pH by 0.3–0.4 units are predicted to affect the carbon physiology and growth of macroalgae. Here we examined how the physiology of the giant kelp Macrocystis pyrifera is affected by elevated pCO2/low pH. Growth and photosynthetic rates, external and internal carbonic anhydrase (CA) activity, HCO3 − versus CO2 use were determined over a 7-day incubation at ambient pCO2 400 µatm/pH 8.00 and a future OA treatment of pCO2 1200 µatm/pH 7.59. Neither the photosynthetic nor growth rates were changed by elevated CO2 supply in the OA treatment. These results were explained by the greater use of HCO3 − compared to CO2 as an inorganic carbon (Ci) source to support photosynthesis. Macrocystis is a mixed HCO3 − and CO2 user that exhibits two effective mechanisms for HCO3 − utilization; as predicted for species that possess carbon-concentrating mechanisms (CCMs), photosynthesis was not substantially affected by elevated pCO2. The internal CA activity was also unaffected by OA, and it remained high and active throughout the experiment; this suggests that HCO3 − uptake via an anion exchange protein was not affected by OA. Our results suggest that photosynthetic Ci uptake and growth of Macrocystis will not be affected by elevated pCO2/low pH predicted for the future, but the combined effects with other environmental factors like temperature and nutrient availability could change the physiological response of Macrocystis to OA. Therefore, further studies will be important to elucidate how this species might respond to the global environmental change predicted for the ocean.

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Acknowledgments

We acknowledge the support of a PhD scholarship from the Chilean government to Pamela A. Fernández (BECAS CHILE-CONICYT) and a grant from The Royal Society of New Zealand Marsden fund (UOO0914) to Catriona L. Hurd. The authors are grateful to Pablo Leal for his help with seaweed collection.

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Affiliations

  1. Department of Botany, University of Otago, PO box 56, Dunedin, 9054, New Zealand

    Pamela A. Fernández, Michael Y. Roleda & Catriona L. Hurd

  2. Bioforsk Norwegian Institute for Agricultural and Environmental Research, Kudalsveien 6, 8049, Bodø, Norway

    Michael Y. Roleda

  3. Institute for Marine and Antarctic Studies (IMAS), University of Tasmania, Hobart, TAS, 7001, Australia

    Catriona L. Hurd

Authors
  1. Pamela A. Fernández
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  2. Michael Y. Roleda
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  3. Catriona L. Hurd
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Correspondence to Pamela A. Fernández.

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Fernández, P.A., Roleda, M.Y. & Hurd, C.L. Effects of ocean acidification on the photosynthetic performance, carbonic anhydrase activity and growth of the giant kelp Macrocystis pyrifera . Photosynth Res 124, 293–304 (2015). https://doi.org/10.1007/s11120-015-0138-5

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Keywords

  • Carbonic anhydrase
  • Inorganic carbon uptake
  • Macroalgae
  • Macrocystis pyrifera
  • Ocean acidification
  • Photosynthesis