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Energy transfer in the chlorophyll f-containing cyanobacterium, Halomicronema hongdechloris, analyzed by time-resolved fluorescence spectroscopies

Photosynthesis Research volume 125, pages 115–122 (2015)Cite this article

Abstract

We prepared thylakoid membranes from Halomicronema hongdechloris cells grown under white fluorescent light or light from far-red (740 nm) light-emitting diodes, and observed their energy-transfer processes shortly after light excitation. Excitation–relaxation processes were examined by steady-state and time-resolved fluorescence spectroscopies. Two time-resolved fluorescence techniques were used: time-correlated single photon counting and fluorescence up-conversion methods. The thylakoids from the cells grown under white light contained chlorophyll (Chl) a of different energies, but were devoid of Chl f. At room temperature, the excitation energy was equilibrated among the Chl a pools with a time constant of 6.6 ps. Conversely, the thylakoids from the cells grown under far-red light possessed both Chl a and Chl f. Two energy-transfer pathways from Chl a to Chl f were identified with time constants of 1.3 and 5.0 ps, and the excitation energy was equilibrated between the Chl a and Chl f pools at room temperature. We also examined the energy-transfer pathways from phycobilisome to the two photosystems under white-light cultivation.

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Abbreviations

Chl:

Chlorophyll

DV-Chl:

3,8-Divinyl chlorophyll

LED:

Light-emitting diodes

PBS:

Phycobilisome

PS:

Photosystem

TRFS:

Time-resolved fluorescence spectrum (spectra)

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Acknowledgments

This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education of Japan (23370013 to SA, 22370017 to SA and TT, and 24370025, 26220801 to TT), a grant from JST PRESTO (TT), and a Grant from the Australian Research Council’s Discovery Projects funding scheme (project number DP12101360 to TT). MC holds an Australian Research Council Future Fellowship (FT120100464), and MC thanks the Australian Research Council for support (DP120100286, CE140100015). SIA was supported by Grants from the Russian Science Foundation (No: 14-14-00039).

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Authors and Affiliations

  1. Molecular Photoscience Research Center, Kobe University, Kobe, 657-8501, Japan

    Seiji Akimoto

  2. CREST, Japan Science and Technology Agency (JST), Kobe, 657-8501, Japan

    Seiji Akimoto

  3. Faculty of Science, Tokyo University of Science, Tokyo, 162-8601, Japan

    Toshiyuki Shinoda & Tatsuya Tomo

  4. ARC Centre of Excellence for Translational Photosynthesis & School of Biological Sciences (A12), University of Sydney, Sydney, NSW, 2006, Australia

    Min Chen

  5. Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, Moscow, 127276, Russia

    Suleyman I. Allakhverdiev

  6. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    Suleyman I. Allakhverdiev

  7. Department of Plant Physiology, Faculty of Biology, M.V. Lomonosov Moscow State University, Leninskie Gory 1-12, Moscow, 119991, Russia

    Suleyman I. Allakhverdiev

  8. PRESTO, Japan Science and Technology Agency (JST), Saitama, 332-0012, Japan

    Tatsuya Tomo

Authors
  1. Seiji Akimoto
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  2. Toshiyuki Shinoda
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  3. Min Chen
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  4. Suleyman I. Allakhverdiev
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  5. Tatsuya Tomo
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Correspondence to Seiji Akimoto.

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Akimoto, S., Shinoda, T., Chen, M. et al. Energy transfer in the chlorophyll f-containing cyanobacterium, Halomicronema hongdechloris, analyzed by time-resolved fluorescence spectroscopies. Photosynth Res 125, 115–122 (2015). https://doi.org/10.1007/s11120-015-0091-3

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  • DOI: https://doi.org/10.1007/s11120-015-0091-3

Keywords

  • Energy transfer
  • Fluorescence
  • Light adaptation
  • Chlorophyll f
  • Pigment-protein complex
  • Time-resolved spectroscopy