Fucoxanthin–chlorophyll proteins (FCP) are the major light-harvesting proteins of diatom algae, a major contributor to marine carbon fixation. FCP complexes from representatives of centric (Cyclotella meneghiniana) and pennate (Phaeodactylum tricornutum) diatoms were prepared by sucrose gradient centrifugation and studied by means of electron microscopy followed by single particle analysis. The oligomeric FCP from a centric diatom were observed to take the form of unusual chain-like or circular shapes, a very unique supramolecular assembly for such antennas. The existence of the often disputed oligomeric form of FCP in pennate diatoms has been confirmed. Contrary to the centric diatom FCP, pennate diatom FCP oligomers are very similar to oligomeric antennas from related heterokont (Stramenopila) algae. Evolutionary aspects of the presence of novel light-harvesting protein arrangement in centric diatoms are discussed.
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Transmission electron microscopy
Polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate
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The authors would like to acknowledge institutional support RVO:60077344 as well as financial support of the Czech Science Foundation projects P205/11/1164 (Z. G.) and P501/12/G055 (R. L., D. B. and F. V.).
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Gardian, Z., Litvín, R., Bína, D. et al. Supramolecular organization of fucoxanthin–chlorophyll proteins in centric and pennate diatoms. Photosynth Res 121, 79–86 (2014). https://doi.org/10.1007/s11120-014-9998-3
- Transmission electron microscopy
- Fucoxanthin–chlorophyll proteins
- Light harvesting
- Thylakoid membrane