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Spectral properties of a divinyl chlorophyll a harboring mutant of Synechocystis sp. PCC6803

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Abstract

A divinyl chlorophyll (DV-Chl) a harboring mutant of Synechocystis sp. PCC 6803, in which chlorophyll species is replaced from monovinyl(normal)-Chl a to DV-Chl a, was characterized. The efficiency of light utilization for photosynthesis was decreased in the mutant. Absorption spectra at 77 K and their fourth derivative analyses revealed that peaks of each chlorophyll forms were blue-shifted by 1–2 nm, suggesting lowered stability of chlorophylls at their binding sites. This was also true both in PSI and PSII complexes. On the other hand, fluorescence emission spectra measured at 77 K were not different between wild type and the mutant. This indicates that the mode of interaction between chlorophyll and its binding pockets responsible for emitting fluorescence at 77 K is not altered in the mutant. P700 difference spectra of thylakoid membranes and PSI complexes showed that the spectrum in Soret region was red-shifted by 7 nm in the mutant. This is a characteristic feature of DV-Chl a. Microenvironments of iron–sulfur center of a terminal electron acceptor of PSI complex, P430, were practically the same as that of wild type.

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Abbreviations

Chl:

Chlorophyll

DDM:

Dodecyl-β-d-maltoside

DV-Chl a :

Divinyl chlorophyll a

DVR:

Divinyl reductase

LHC:

Light-harvesting complex

MES:

2-Morpholinoethanesulfonic acid

MV-Chl a :

Monovinyl chlorophyll a

PMS:

Phenazine methosulphate

PSI and II:

Photosystem I and II

Synechocystis 6803:

Synechocystis sp. PCC6803

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Acknowledgments

This work was supported in part by a grant of 21st century Center of Excellence Program to KS and by Chuo University Grant for Special Research to HK.

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Author notes

  1. Md. Rafiqul Islam

    Present address: Department of Cell Physiology, National Institute for Physiological Sciences, Okazaki, Aichi, 444-8787, Japan

Authors and Affiliations

  1. Department of Life Science, Graduate School of Life Science, University of Hyogo, Ako, Hyogo, 678-1297, Japan

    Md. Rafiqul Islam, Yasuhiro Kashino & Kazuhiko Satoh

  2. Department of Life Sciences, Faculty of Science and Engineering, Chuo University, Kasuga, Bunkyo-ku, 112-8551, Japan

    Koji Watanabe & Hiroyuki Koike

Authors
  1. Md. Rafiqul Islam
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  2. Koji Watanabe
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  3. Yasuhiro Kashino
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  4. Kazuhiko Satoh
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  5. Hiroyuki Koike
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Corresponding author

Correspondence to Hiroyuki Koike.

Additional information

This article is also dedicated to Professor Gernot Renger who passed away on 12th January 2013.

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Islam, M.R., Watanabe, K., Kashino, Y. et al. Spectral properties of a divinyl chlorophyll a harboring mutant of Synechocystis sp. PCC6803. Photosynth Res 117, 245–255 (2013). https://doi.org/10.1007/s11120-013-9877-3

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