Abstract
The temporal profile of the phosphorescence of singlet oxygen endogenously photosensitized by photosystem II (PSII) reaction centre (RC) in an aqueous buffer has been recorded using laser excitation and a near infrared photomultiplier tube. A weak emission signal was discernible, and could be fitted to the functional form \( a[\exp ( - t/\tau_{2} ) - \exp ( - t/\tau_{1} )] \), with \( a > 0 \) and \( \tau_{2} > \tau_{1} \). The value of \( \tau_{2} \) decreased from 11.6 ± 0.5 μs under aerobic conditions to 4.1 ± 0.2 μs in oxygen-saturated samples, due to enhanced bimolecular quenching of the donor triplet by oxygen, whereas that of \( \tau_{1} \), identifiable with the lifetime of singlet oxygen, was close to 3 μs in both cases. Extrapolations based on the low amplitude of the emission signal of singlet oxygen formed by PSII RC in the aqueous buffer and the expected values of \( \tau_{1} \) and \( \tau_{2} \) in chloroplasts indicate that attempts to analyse the temporal profile of singlet oxygen in chloroplasts are unlikely to be rewarded with success without a significant advance in the sensitivity of the detection equipment.
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Acknowledgments
This study was funded by the Research Council of Norway (Project No. 191102) and the Spanish Ministry of Science and Innovation (Grant number BFU2007-68107-C02-02/BMC). JBA is very grateful to the CSIC mobility programme for funding his short term visit to NTNU (Ref. number PA1002668). We thank Mr. José J. Martín for technical assistance.
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Li, H., Melø, T.B., Arellano, J.B. et al. Temporal profile of the singlet oxygen emission endogenously produced by photosystem II reaction centre in an aqueous buffer. Photosynth Res 112, 75–79 (2012). https://doi.org/10.1007/s11120-012-9739-4
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DOI: https://doi.org/10.1007/s11120-012-9739-4