A tribute to Thomas Roosevelt Punnett, Jr. (1926–2008)
- 590 Downloads
We honor here Thomas (Tom) Roosevelt Punnett, Jr. (May 25, 1926–July 4, 2008), who was a pioneer of Biology, particularly of biochemistry of plants and algae, having specialized in photosynthesis under Robert Emerson of the University of Illinois at Urbana-Champaign. He did exciting work on regulation and control of various metabolic reactions. He was an innovator and raconteur par excellence, and he prized critical thinking. His enthusiasm for basic science questions was matched by his grasp of their “real-world” implications. His last project was a patent for anaerobic sewage treatment that he hoped would lead to solution of waste disposal and energy creation world wide, including the clean-up of Lake Erie, where he had sailed as a boy. On the personal side, he had a strong sense of morality and a great wit and humor.
KeywordsChlorella pyrenoidosa Elodea Robert Emerson Robin Hill Leukocyte Methane production from organic material Eugene Rabinowitch Temple University University of Illinois at Urbana-Champaign University of Rochester Yale University
Early life and education
Tom entered Yale University after his discharge from the army in 1946, receiving his B.S. in Chemistry in 1950. That same year he married Hope Handler, whom he had met at Yale where she was a graduate student in Genetics.
Tom enrolled in the Graduate College of the University of Illinois at Urbana-Champaign in September of 1950, and worked in the laboratory of Robert (Bob) Emerson. Besides Emerson, his doctoral committee included Eugene Rabinowitch (physical chemist), Sol Spiegelman (microbiologist), R.D. Rawcliffe (physicist), Carl S. Vestling (biochemist), and I.C. (Gunny) Gunsalus (biochemist). This was an outstanding group of scholars for a young research plant biologist to train with. Even before his doctoral thesis, Tom published a paper in Nature on oxygen evolution in algal chloroplast (Punnett and Fabiye 1953). This provided the first direct method for comparing the activities in vitro and in vivo of the photochemical systems in Chlorella, an organism widely used in the study of in vivo photosynthetic reactions.
Marcia Brody, who had later joined Emerson’s lab, recently told one of us (Govindjee): “When I came to Emerson’s lab, Tom was there. He was extremely kind, and could not have been kinder; he was very helpful to me; he was a thoughtful and a generous person, and had a good sense of humor. He was a very dedicated scientist.”
I remember [Tom and Hope Punnett] from my days at the University of Illinois in the 1950s rather well. We all used to play bridge in their house, on hot summer days in Urbana. Tom was a most friendly, helpful co-worker in Emerson’s lab. Emerson thought very highly of him. Emerson had recommended that Tom do a post-doc in England with Robin Hill, and he did go to Cambridge for that.
(See a Tribute to Steve Brody by Hirsch et al. 2010.)
Academic and research life
After receiving his Ph.D. (Punnett 1954), Tom began a post-doctoral fellowship in biochemistry at Cambridge University, England, where he worked with Robert (Robin) Hill on the influence of environmental conditions on photosynthesis. At this time no one knew about the two photosystems in plants (for Timeline of discoveries in photosynthesis, see Govindjee and Krogmann 2004). Hill (1937, 1939) had found that the oxygen evolving part of photosynthesis could be separated from the carbon dioxide fixation part by using an electron acceptor such as ferricyanide. Tom combined this “Hill” reaction and his own experience with the single cell alga, Chlorella pyrenoidosa. Chlorella was the organism of choice as it was easy to grow and could be used directly in gas exchange measurements using Warburg manometers or oxygen electrodes.
Tom and Hope were very kind to us when we were on sabbatical in Philadelphia 1968–1969 with a young family. He always seemed to be brimming over with a youthful enthusiasm for science and for his experiments, despite suffering at least his fair share of difficulties and setbacks..What Tom had been doing for his Ph.D. with Emerson fitted very well with one of Robin Hill’s main research interests at the time-natural electron acceptors…., except that Tom had been working mainly with Chlorella…. Robin got him to spend much of his time with plant material….
Returning to the United States in 1956, Tom joined the faculty of the University of Rochester where he stayed for 7 years. His research efforts were focused primarily in photosynthesis, but he also published a paper with his wife, Hope (one of the authors of this Tribute), in Nature, on a leukocyte growth factor isolated from red beans (Punnett and Punnett 1963; Punnett et al. 1962). Later, Punnett et al. (1980) did an analysis of hydrozoan sperm attractant. His understanding of biochemical techniques including processes for the purification of proteins was exceptional.
The primary focus of Tom’s research life remained an unquenchable interest in photosynthesis, stemming from the early experiments of Robert Emerson on photosynthetic processes in plants. Emerson and Lewis (1943) had found that the quantum yield of photosynthesis dropped precipitously when algae were illuminated beyond 685 nm (the so-called Red Drop). A major breakthrough came when Emerson et al. (1957) discovered a synergistic effect by illuminating algae with two beams together, one in the red drop region and another on the short-wave side of the spectrum. This phenomenon, now known as the Emerson Enhancement Effect, implied that there were two photosystems involved in the photosynthetic process. Emerson’s enhancement experiment was the seminal experiment for establishing the two light system hypothesis in plant photosynthesis (also see Govindjee and Rabinowitch 1960; Myers and French 1960). During this period, Punnett (1959) continued his experiments with broken chloroplasts along with their uncertainties, and this moved him toward techniques for proper isolation of chloroplasts.
Tom was very much interested in the environmental factors that might influence in vivo and subsequent in vitro results with the isolation of chloroplasts. He became interested in plant growth conditions prior to photosynthesis measurements with either intact plants or isolated chloroplasts. One of his research papers from the Temple University showed that growth conditions of the plant resulted in differences in enhancement of photophosphorylation by CO2 (Punnett 1965). This experiment set his research direction for the next few years. He soon presented his paper on isolation of non-granular chloroplasts from higher plants (Punnett 1966).
Tom started to work again with C. pyrenoidosa to study the changes in development and photosynthesis that occur during the life cycle of this alga. Punnett and Derrenbacker (1966) described the aminoacid composition of algal cell walls. He and one of us (Hagar) developed synchronization techniques to have most of the cultured cells complete their life cycle in 24 h; thus, they were able to look at developmental stages a few hours apart and to monitor the in vivo changes in pigment protein compositions while they measured photosynthetic rates of the cell culture. They also described the synchronization process and the unique use of Probit Analysis to better follow and characterize cell synchrony (Hagar and Punnett 1973).
During this time, Tom also focused on the aquarium plant, Elodea, to investigate the relationship between in vivo and in vitro measurements. He was especially intrigued with literature reports of granular or homogenous chloroplasts and the isolation of such “intact” chloroplasts (Sager and Palade 1956). He found that pretreatment of Elodea with red or blue light would cause a change in the observable chloroplast structure. With red light, he could push the plant into a more homogeneous state where granular stacks could not be observed. He developed the methodology to isolate chloroplasts with visible grana stacks. Punnett et al. (1981) reported that chloroplasts undergo rapid rearrangements in vivo. By this time it was known that there were two photosystems connected by an electron transport chain. Tom found that the Emerson Enhancement effect was not observed under conditions when the two photosystems are well balanced; the effect is seen only when there is an unbalanced excitation of the systems (Punnett 1970). This is a very important observation because lack of Emerson Enhancement must never be taken as evidence of the absence of two light reactions and two photosystems.
Tom extended the work on Elodea to demonstrate that the sensitivity of chloroplast structure to environmental conditions, as observed by both light and electron microscopy, was also present in terrestrial plants (Punnett and Kelly 1975, 1976). This transformation was achieved with plants from nine different genera, including both monocotyledonous and dicotyledonous plants with either Kranz or conventional leaf anatomy.
Tom’s homogeneous chloroplast did not show the Emerson enhancement effect, but his grana stack chloroplasts did show it for the first series of measurements. Thus, Tom was able to prepare intact plants that would exhibit differences in enhancement due to different states of plant tissue. This may be related to the so-called State changes studied by Bonaventura and Myers (1969) and Murata (1969), among others (see a historical review on state changes by Papageorgiou and Govindjee 2011).
The other control mechanism that Tom worked on was the problem of metabolites that could not be accounted for by either the Calvin-Benson cycle or ß-carboxylation. Thus, his two major research directions, both involving work on control mechanisms, led Tom to find himself in a position of confronting scientific orthodoxy, which he had not set out to do.
Tom postulated that the unexplained metabolites indicated the existence of two or more CO2 fixation pathways, whose relative rates are controlled by environmental conditions. Again, wavelength and intensity of light and humidity were shown to modify these pathways. His work involved the search for the quantifiable and reproducible conditions under which these proposed pathways could occur. This led him to an interest in learning the technique of photoacoustic spectroscopy, as developed by Shmuel Malkin (Weizmann Institute). Tom worked as a Meyerhof Fellow, in Malkin’s laboratory, where he was able to investigate his hypothesis concerning the reverse Krebs cycle. However, he was frustrated by his inability to identify all of the necessary environmental conditions and to reproduce the same result after he returned to Temple University, where he had less than ideal conditions for growing plants.
…. [Tom] came to me to learn all about the photoacoustic method and its use in photosynthesis. He was a very social and enthusiastic person. He was very happy about the method and the information that it gave him about the photosynthesis of palisade mesophyll vs. spongy mesophyll cells. He used to walk in the lab and talk about it very loudly and proudly. I remember him showing me how he grows plants, the special soil that he had designed for the plants and how the growth conditions affected photosynthesis (from the photoacoustic results)…
(See Punnett (1987) that describes his work with photoacoustic spectroscopy in understanding environmental control of photosynthetic enhancement.)
Teaching, his collaborative spirit, and who he really was
At Temple, many students relied on the affable and talented professor not only in his own laboratory but also in other laboratories, when faculty members left for one reason or another. A former student, Howard Nields, put it, “..we were orphans of the storm, and Tom Punnett graciously became our foster father, so to speak.” Tom took on this advising with compassion, critical scientific input, and unbridled energy. He redirected some graduate students toward more promising research endeavors, and he guided others to successful careers in academic settings. His record during this cohort cycle was six doctoral theses.
Tom collaborated with colleagues in Chemistry to develop an inter-departmental Biochemistry program, which he directed for a number of years. He worked with fellow faculty members and students to solve a wide range of problems from purifying sperm attractants from starfish (Punnett et al. 1992) to comparing chlorophyll protein complexes of plants and photosynthetic bacteria for environmental control of photosynthesis (Webb and Punnett 1989). He was a visiting professor at University College, London, U.K. (1968–1969), spent one sabbatical at the Research Institute for Advanced Studies (RIAS) in Baltimore with Bessel Kok (1961), another leave at the Weizmann Institute in Rehovot, Israel (1986), as mentioned above, and his last at the US Department of Agriculture (USDA) in Beltsville, MD (1991).
Tom enjoyed his students and he loved teaching, which was not a rote activity; he never gave the same lecture twice. He communicated the scientific process as a series of trials and errors undertaken by fallible human beings. Biographical information about the researchers whose work he discussed enlivened his lectures. He prized critical thinking and was careful to make sure his students solved their own scientific problems. He instilled the ability to see multiple viewpoints and ask the pertinent questions.
To his students, Tom Punnett was an innovator and a captivating lecturer. His wicked wit was as evident as his strong sense of morality. He was a caring mentor, helping his students with everything from language skills to job and graduate school applications. Those completing their doctorates with him went on to successful scientific careers, often using his teaching techniques to stimulate students of their own. He encouraged undergraduate students to join his research group. He took them to scientific meetings, along with graduate students, where they had the opportunity to hear results challenged and theories debated. He knew his students’ families and he enjoyed entertaining them at home.
Tom’s enthusiasm for basic science questions was matched by his grasp of their “real-world” implications. Only a year before he died, he had applied for a patent (International Publication Number WO 2008/002448 A2: A method of maximizing methane production from organic material) to optimize anaerobic metabolism of municipal wastes. The process has the potential to greatly diminish solid waste while leading to high production of economically valuable methane. Additional benefits would be an increase in the purity of sewage plant output discharged into receiving waters, reduction of CO2 released to the atmosphere when biologically generated methane is used as fuel and production of a final sludge that, when pasteurized, could be used as a nutritious soil additive. Unfortunately, he did not live to complete the experimental validation procedures.
Although he retired from teaching when health problems made it necessary, he continued his research until his death. His never failing force of will and sense of humor enabled him to keep going. He stayed abreast of developments in the field, attending Gordon Conferences and international meetings. In 2005, his scientific colleagues recognized him when they asked him to chair the Eastern Regional Photosynthesis Conference. His choice of invited speakers gave evidence of how closely he followed seminal research efforts in the area. He attended all but one of the Eastern Regional Photosynthesis Conferences during the past 25 years including the meeting in 2008, just a few weeks before his final illness.
Tom Punnett was a history and archeology buff, an avid connoisseur of classical music, and an enthusiastic gardener. He grew up sailing on Lake Erie, which inspired a life-time passion both for sailing and for the natural environment. Combined with his scientific interests, these led him to an early appreciation of ecology and the need for environmental protection. In the days of the Cold War and nuclear threat, he helped to found the Rochester Committee for Scientific Information, an early environmental action and study group. In Philadelphia he was active in the Sierra Club, providing technical information on issues such as water quality.
We thank George C. Papageorgiou, who knew several members of the Emerson-Rabinowitch Photosynthesis Project, for his valuable suggestions, and for editing the final copy of this manuscript. George was our guest editor, who enthusiastically recommended acceptance of this Tribute for publication in Photosynthesis Research.
- Bonaventura C, Myers J (1969) Fluorescence and oxygen evolution from Chlorella pyrenoidosa. Biochim Biophys Acta 89:366–383Google Scholar
- Govindjee, Rabinowitch E (1960) Two forms of chlorophyll a in vivo with distinct photochemical functions. Science 132:159–160Google Scholar
- Govindjee, van Rensen JJS (1978) Bicarbonate effects on the electron flow in isolated broken chloroplasts. Biochim Biophys Acta 505:183–213Google Scholar
- Punnett T (1987) Environmental control of photosynthetic enhancement in palisade mesophyll chloroplasts measured by photoacoustic spectroscopy. In: Biggins J (ed) Progress in photosynthesis research, vol 2. Nijhoff, Dordrecht, pp 753–756Google Scholar
- Punnett T and Kelly JH (1975). Ultrastructural transformation of chloroplasts in terrestrial plants. J Cell Biol; see Meeting Supplement abstract book; not available online, p 346aGoogle Scholar
- Punnett T and Kelly JH (1976) Environmental control over C3 and photosynthesis in vascular plants. Plant Physiol (May 1976 Annual Meeting Supplement), p. 305Google Scholar
- Punnett T, Miller RL, Webb R (1980) Reanalysis of the chemical properties of two hydrozoan sperm attractants. Am Zool 20:833Google Scholar
- Punnett T, Hilfer SR and J. Brown (1981). Chloroplast thylakoid undergo rapid rearrangements in vivo J Cell Biol; see Meeting Supplement abstract book; not available on line, p 287aGoogle Scholar
- Rabinowitch E (1961) Robert Emerson (1903–1959). In: Biographical Memoirs, vol 25. National Academy of Sciences, Washington, DC, pp 112–131Google Scholar