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Increase in the activity of fructose-1,6-bisphosphatase in cytosol affects sugar partitioning and increases the lateral shoots in tobacco plants at elevated CO2 levels

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Abstract

We generated transgenic tobacco plants with high levels of fructose-1,6-bisphosphatase expressing cyanobacterialfructose-1,6-/sedoheptulose-1,7-bisphosphatase in the cytosol. At ambient CO2 levels (360 ppm), growth, photosynthetic activity, and fresh weight were unchanged but the sucrose/hexose/starch ratio was slightly altered in the transgenic plants compared with wild-type plants. At elevated CO2 levels (1200 ppm), lateral shoot, leaf number, and fresh weight were significantly increased in the transgenic plants. Photosynthetic activity was also increased. Hexose accumulated in the upper leaves in the wild-type plants, while sucrose and starch accumulated in the lower leaves and lateral shoots in the transgenic plants. These findings suggest that cytosolic fructose-1,6-bisphosphatase contributes to the efficient conversion of hexose into sucrose, and that the change in carbon partitioning affects photosynthetic capacity and morphogenesis at elevated CO2 levels.

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Abbreviations

F2,6P2 :

Fructose 2,6-bisphosphate

F6P:

Fructose 6-phosphate

FBP:

Fructose 1,6-bisphosphate

FBPase:

Fructose-1,6-bisphosphatase

FBP/SBPase:

Fructose-1,6-/sedoheptulose-1,7-bisphosphatase

PFK:

6-Phosphofructokinase

PFP:

Fructose-6-phosphate 1-phosphotransferase

Pi:

Inorganic phosphate

PPi:

Pyrophosphate

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Acknowledgment

This research was supported by CREST, JST.

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Authors and Affiliations

  1. Department of Advanced Bioscience, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara, 631-8505, Japan

    Masahiro Tamoi, Yoshie Hiramatsu, Shigeki Nedachi, Kumi Otori, Noriaki Tanabe, Takanori Maruta & Shigeru Shigeoka

  2. Japan Science and Technology Agency (JST), Core Research for Evolutional Science and Technology (CREST), Kawaguchi, 332-0012, Japan

    Masahiro Tamoi, Kumi Otori, Noriaki Tanabe, Takanori Maruta & Shigeru Shigeoka

Authors
  1. Masahiro Tamoi
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  2. Yoshie Hiramatsu
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  3. Shigeki Nedachi
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  4. Kumi Otori
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  5. Noriaki Tanabe
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  6. Takanori Maruta
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  7. Shigeru Shigeoka
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Correspondence to Shigeru Shigeoka.

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Tamoi, M., Hiramatsu, Y., Nedachi, S. et al. Increase in the activity of fructose-1,6-bisphosphatase in cytosol affects sugar partitioning and increases the lateral shoots in tobacco plants at elevated CO2 levels. Photosynth Res 108, 15–23 (2011). https://doi.org/10.1007/s11120-011-9645-1

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  • DOI: https://doi.org/10.1007/s11120-011-9645-1

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