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Desiccation of the resurrection plant Haberlea rhodopensis at high temperature

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Abstract

Haberlea rhodopensis plants, growing under low irradiance in their natural habitat, were desiccated to air-dry state at a similar light intensity (about 30 μmol m−2 s−1) under optimal (23/20°C, day/night) or high (38/30°C) temperature. Dehydration of plants at high temperature increased the rate of water loss threefold and had a more detrimental effect than either drought or high temperature alone. Water deficit decreased the photochemical activity of PSII and PSI and the rate of photosynthetic oxygen evolution, and these effects were stronger when desiccation was carried out at 38°C. Some reduction in the amount of the main PSI and PSII proteins was observed especially in severely desiccated Haberlea leaves. The results clearly showed that desiccation of the homoiochlorophyllous poikilohydric plant Haberlea rhodopensis at high temperature had more damaging effects than desiccation at optimal temperature and in addition recovery was slower. Increased thermal energy dissipation together with higher proline and carotenoid content in the course of desiccation at 38°C compared to desiccation at 23°C probably helped in overcoming the stress.

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Abbreviations

PSII:

Photosystem II

PSI:

Photosystem I

MDA:

Malondialdehyde

RWC:

Relative water content

Chl:

Chlorophyll

Fv/Fm:

Maximal quantum efficiency of photosystem II in the dark adapted state

ΦPSII:

Quantum yield of PSII electron transport in the light-adapted state

LNU:

Proportion of light not used for photochemistry

References

  • Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216

    Article  CAS  Google Scholar 

  • Bartels D (2005) Desiccation tolerance studied in the resurrection plant Craterostigma plantagineum. Integr Comp Biol 45:696–701

    Article  CAS  Google Scholar 

  • Barua D, Heckathorn SA (2006) The interactive effects of light and temperature on heat shock protein accumulation in Solidago altissima (Asteraceae) in the field and laboratory. Am J Bot 93:102–109

    Article  CAS  Google Scholar 

  • Bates LS, Waldren RP, Teare JD (1973) Rapid determination of proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bewley JD, Oliver MJ (1992) Desiccation tolerance in vegetative tissues and seeds: protein synthesis in relation to desiccation and a potential role for protection and repair mechanism. In: Osmond CB, Somero G (eds) Water and life: a comparative analysis of water relationships at the organismic, cellular and molecular levels. Springer, Berlin, pp 141–160

    Google Scholar 

  • Boucher N, Carpentier R (1993) Heat-stress stimulation of oxygen uptake by Photosystem I involves the reduction of superoxide radicals by specific electron donors. Photosynth Res 35:213–218

    Article  CAS  Google Scholar 

  • Di Blasi S, Puliga S, Losi L, Vazzana C (1998) S. stapfianus and E. curvula cv. Consol in vivo photosynthesis, PSII activity and ABA content during dehydration. Plant Growth Regulation 25:97–104

    Article  CAS  Google Scholar 

  • Drozdova I, Pustovoitova T, Dzhibladze T, Barabanshchikova N, Zhdanova N, Maevskaya S, Bukhov N (2004) Endogenous control of photosynthetic activity during progressive drought: influence of final products of photosynthesis. Russ J Plant Physiol 51:742–750

    Article  Google Scholar 

  • Esterbauer H, Cheeseman KH (1990) Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 186:407–421

    Article  PubMed  CAS  Google Scholar 

  • Farrant JM, Sherwin HS (1998) Mechanisms of desiccation tolerance in seeds and resurrection plants. In: Taylor AG, Huang X-L (eds) Progress in seed research. Proceedings of 2nd international conference on seed science and technology, Geneva, NY. Communication Services of New York State Agricultural Experiment Station, pp 109–120

  • Farrant JM, Cooper K, Kruger LA, Sherwin HW (1999) The effect of drying rate on the survival of three desiccation-tolerant angiosperm species. Ann Bot 84:371–379

    Article  Google Scholar 

  • Feller U, Crafts-Brandner SJ, Salvucci ME (1998) Moderately high temperatures inhibit ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) activase-mediated activation of rubisco. Plant Physiol 116:539–546

    Article  PubMed  CAS  Google Scholar 

  • Frank HA, Young AJ, Britton G, Cogdell RJ (1999) The photochemistry of carotenoids. In: Govindjee (ed) Advances in photosynthesis, vol 8. Kluwer, Dordrecht

    Google Scholar 

  • Gaff DF (1971) Desiccation tolerant flowering plants in Southern Africa. Science 174:1033–1034

    Article  PubMed  CAS  Google Scholar 

  • Georgieva K, Maslenkova L (2006) Thermostability and photostabitity of PSII in leaves of resurrection plant Haberlea rhodopensis studied by means of chlorophyll fluorescence. Z Naturforsch 61c:234–240

    Google Scholar 

  • Georgieva K, Maslenkova L, Peeva P, Markovska Yu, Stefanov D, Tuba Z (2005) Comparative study on the changes in photosynthetic activity of the homoiochlorophyllous desiccation-tolerant Haberlea rhodopensis and desiccation-sensitive spinach leaves during desiccation and rehydration. Photosynth Res 85:191–203

    Article  PubMed  CAS  Google Scholar 

  • Georgieva K, Szigeti Z, Sarvari E, Gaspar L, Maslenkova L, Peva V, Peli E, Tuba Z (2007) Photosynthetic activity of homoiochlorophyllous desiccation tolerant plant Haberlea rhodopensis during desiccation and rehydration. Planta 225:955–964

    Article  PubMed  CAS  Google Scholar 

  • Havaux M, Tardy F (1999) Loss of chlorophyll with limited reduction of photosynthesis as an adaptive response of Syrian barley landraces to high light and heat stress. Aust J Plant Physiol 26:569–578

    Article  CAS  Google Scholar 

  • Klughammer C, Schreiber U (1998) Measuring P700 absorbance changes in the near infrared spectral region with a dual wavelength pulse modulation system. In: Garab G (ed) Photosynthesis: mechanisms and effects, vol V. Kluwer, Dordrecht, pp 4357–4360

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids—the pigments of photosynthetic biomembranes. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 148. Academic Press, San Diego, pp 350–382

  • Lu C, Zhang J (1999) Effects of water stress on photosystem II photochemistry and its thermostability in wheat plants. J Exp Bot 50:1199–1206

    Article  CAS  Google Scholar 

  • Oliver M, Tuba Z, Mishler B (2000) The evolution of vegetative desiccation tolerance in land plants. Plant Ecol 151:85–100

    Article  Google Scholar 

  • Reddy A, Chaitanya K, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189–1202

    Article  CAS  Google Scholar 

  • Rizhsky L, Liang H, Shuman J, Shulaev V, Davletova S, Mittler R (2004) When defense pathways collide. The response of arabidopsis to a combination of drought and heat stress. Plant Physiol 134:1683–1696

    Article  PubMed  CAS  Google Scholar 

  • Saccardy K, Pineau B, Roche O, Cornic G (1998) Photochemical efficiency of photosystem II and xanthophyll cycle components in Zea mays leaves exposed to water stress and high light. Photosynth Res 56:57–66

    Article  CAS  Google Scholar 

  • Sayed OH, Earnshaw MJ, Emes MJ (1989) Photosynthetic response of different varieties of wheat to high temperature. II. Effect of heat stress on photosynthetic electron transport. J Exp Bot 40:633–638

    Article  CAS  Google Scholar 

  • Schägger H, von Jagov G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166:368–379

    Article  PubMed  Google Scholar 

  • Tyystjarvi E, Karunen J (1990) A microcomputer program and fast analog to digital converter card for the analysis of fluorescence induction transients. Photosynth Res 26:27–132

    Google Scholar 

  • Zivkovic T, Quartacci M, Stevanovic B, Marinone F, Navari-Izzo F (2005) Low-molecular weight substances in the poikilohydric plant Ramonda serbica during dehydration and rehydration. Plant Sci 168:105–111

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Science Fund [Project D002-208/2008] and the Deutsche Forschungsgemeinschaft (Bu812/6-1).

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Authors and Affiliations

  1. Acad. M. Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113, Sofia, Bulgaria

    Gergana Mihailova, Snejana Petkova & Katya Georgieva

  2. Institute of Molecular Biosiences, University of Frankfurt, Siesmayerstrasse 70, 60323, Frankfurt, Germany

    Claudia Büchel

Authors
  1. Gergana Mihailova
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  2. Snejana Petkova
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  3. Claudia Büchel
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  4. Katya Georgieva
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Correspondence to Katya Georgieva.

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Mihailova, G., Petkova, S., Büchel, C. et al. Desiccation of the resurrection plant Haberlea rhodopensis at high temperature. Photosynth Res 108, 5–13 (2011). https://doi.org/10.1007/s11120-011-9644-2

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  • DOI: https://doi.org/10.1007/s11120-011-9644-2

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