Photosynthesis Research

, Volume 105, Issue 1, pp 27–37 | Cite as

Selenium-induced protection of photosynthesis activity in rape (Brassica napus) seedlings subjected to cadmium stress. Fluorescence and EPR measurements

  • Maria FilekEmail author
  • Janusz Kościelniak
  • Maria Łabanowska
  • Elżbieta Bednarska
  • Ewa Bidzińska
Regular Paper


Fluorescence and electron paramagnetic resonance measurements were used to study selenium influence on photosystem activity in rape seedlings affected by Cd stress. Water cultures containing Hoagland nutrients were supplemented with 400 μM of CdCl2, 2 μM of Na2SeO4 and a mixture of both CdCl2 and Na2SeO4. The seedlings were cultured till the first leaf reached about 1 cm in length. Cadmium-induced changes in the activity of both photosystems were partly diminished by Se presence in the nutrient medium. Electron microscopy photographs confirmed less degradation in chloroplasts of plants cultured on media containing Se. It is suggested that sucrose groups of starch, which is deposited in greater amounts in Cd-stressed plants, may act as traps for free radicals produced under those conditions.


Fluorescence EPR Photosystems Cadmium Selenium Rape plants 



Constant of hyperfine splitting (HFS)


Light energy absorbed by leaf cross-section (CS).


Density-functional theory


Probability that an absorbed photon will move an electron into the electron transport chain


Quantum yield of photosynthetic electron transport chain after QA per CS


Efficiency with which a trapped exciton can move an electron into the electron transport chain


Fluorescence with all PSII reaction centres closed in light-exposed leaves


Fluorescence in leaves previously exposed to light, darkened just before measurement


Steady-state fluorescence in light-exposed leaves


PS2 antenna trapping efficiency


Variable fluorescence in light-adapted leaves (Fv′ = Fm′ − Fo′)


Values of g tensor


Non-photochemical quenching of chlorophyll a fluorescence


Fraction of O2 evolving centres PS2 in comparison with the control sample


Performance index, defined on a cross section (CS) basis


Photosynthetic photon flux density


Photosystem II


The first stable electron acceptor in PS2


Photochemical quenching of chlorophyll a fluorescence


Number of active reaction centres (RC) in the state of fully reduced PS2 RC


The working integral of the energy needed to close all RCs


Expresses the average (av) redox state (QA /QA) in the time span from 0 to Fmax and therefore, the average fraction of open (op) RC during the time needed to complete the closure (cl) of all the RC; Sm/t Fmax = [RCop/(RCcl + RCop)]av


Maximum quantum yield of primary photochemistry


Quantum yield of primary photochemistry (from RC to QA) per CS


Quantum yield of PS2 photochemistry


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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Maria Filek
    • 1
    • 2
    Email author
  • Janusz Kościelniak
    • 3
  • Maria Łabanowska
    • 4
  • Elżbieta Bednarska
    • 5
  • Ewa Bidzińska
    • 4
  1. 1.Institute of Plant PhysiologyPolish Academy of SciencesKrakowPoland
  2. 2.Institute of BiologyPedagogical UniversityKrakowPoland
  3. 3.Department of Plant PhysiologyAgriculture UniversityKrakowPoland
  4. 4.Faculty of ChemistryJagiellonian UniversityKrakowPoland
  5. 5.Institute of Cell BiologyM. Copernicus UniversityTorunPoland

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