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Quantitative analysis of the relationship between induction kinetics of chlorophyll fluorescence and function of genes in the cyanobacterium Synechocystis sp. PCC 6803

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Abstract

We developed here the quantitative and objective method to analyze chlorophyll fluorescence from the cyanobacterium Synechocystis sp. PCC 6803 in the aim of systematic examination of gene function. The overall similarity of the chlorophyll fluorescence induction kinetics was evaluated for 499 mutants. Mutants of 333 genes showed the difference in the fluorescence kinetics from that of wild type, indicating the wide interaction of photosynthesis with other metabolisms. Hierarchical clustering of the similarity of the mutants enables us to group together the mutants having defect in the regulation of photosystem stoichiometry as well as those having defects in respiration or other functions. Furthermore, wild-type cells treated with inhibitors of respiration and mutants of genes involved in respiration shared similar induction kinetics. Apparently, quantitative comparison of the induction kinetics could be useful to analyze the function of genes as well as to predict the target sites of various chemicals.

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Abbreviations

PCC:

Pasteur culture collection

CCD:

Charge-coupled device

TES:

N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid

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Acknowledgements

The authors thank to the staff of Kazusa DNA Research Institute for helpful discussion. This work was supported by Grant-in-Aid for Genome Biology from the Ministry of Education, Culture, Sports, Science and Technology to K.S.

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Authors and Affiliations

  1. Kazusa Research Base for Global Environment, Graduate School of Frontier Sciences, The University of Tokyo, 2-6-7 Kazusakamatari, Kisarazu-shi, Chiba, 292-0818, Japan

    Hiroshi Ozaki

  2. Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba, 277-8562, Japan

    Kintake Sonoike

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  1. Hiroshi Ozaki
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Correspondence to Kintake Sonoike.

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Ozaki, H., Sonoike, K. Quantitative analysis of the relationship between induction kinetics of chlorophyll fluorescence and function of genes in the cyanobacterium Synechocystis sp. PCC 6803. Photosynth Res 101, 47–58 (2009). https://doi.org/10.1007/s11120-009-9462-y

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