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Identification of novel genes putatively involved in the photosystem synthesis of Bradyrhizobium sp. ORS 278

Photosynthesis Research volume 100, pages 97–105 (2009)Cite this article

Abstract

In aerobic anoxygenic phototrophs, oxygen is required for both the formation of the photosynthetic apparatus and an efficient cyclic electron transfer. Mutants of Bradyrhizobium sp. ORS278 affected in photosystem synthesis were selected by a bacteriochlorophyll fluorescence-based screening. Out of the 9,600 mutants of a random Tn5 insertion library, 50 clones, corresponding to insertions in 28 different genes, present a difference in fluorescence intensity compared to the WT. Besides enzymes and regulators known to be involved in photosystem synthesis, 14 novel components of the photosynthesis control are identified. Among them, two genes, hsIU and hsIV, encode components of a protein degradation complex, probably linked to the renewal of photosystem, an important issue in Bradyrhizobia which have to deal with harmful reactive oxygen species. The presence of homologs in non-photosynthetic bacteria for most of the regulatory genes identified during study suggests that they could be global regulators, as the RegA–RegB system.

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Abbreviations

AAP:

Aerobic anoxygenic phototroph

Bchl:

Bacteriochlorophyll

CDS:

Coding sequense

LH:

Light-harvesting

PHA:

Polyhydroxyalkanoates

RC:

Photochemical reaction center

Rb. :

Rhodobacter

Rps. :

Rhodopseudomonas

RR:

Response regulator

SD:

Standard deviation

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Acknowledgments

M. J. is indebted to the “Ministère de l’Education Nationale, de l’Enseignement Supérieur et de la Recherche” for a doctoral grant.

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Authors and Affiliations

  1. Laboratoire des Symbioses Tropicales et Méditerranéennes, IRD, CIRAD, AGRO-M, INRA, UM2. TA A-82/J, Campus de Baillarguet, 34398, Montpellier Cedex 5, France

    Marianne Jaubert, Laure Hannibal, Joël Fardoux & Eric Giraud

  2. CEA, DSV, IBEB, Lab Bioenerget Cellulaire, 13108, Saint-Paul-lez-Durance, France

    André Verméglio

  3. CNRS, UMR Biol Veget & Microbiol Environ, 13108, Saint-Paul-lez-Durance, France

    André Verméglio

  4. Aix-Marseille Université, 13108, Saint-Paul-lez-Durance, France

    André Verméglio

Authors
  1. Marianne Jaubert
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  2. Laure Hannibal
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  3. Joël Fardoux
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  4. Eric Giraud
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  5. André Verméglio
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Corresponding author

Correspondence to André Verméglio.

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Eric Giraud and André Verméglio should be considered co-senior authors.

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Jaubert, M., Hannibal, L., Fardoux, J. et al. Identification of novel genes putatively involved in the photosystem synthesis of Bradyrhizobium sp. ORS 278. Photosynth Res 100, 97–105 (2009). https://doi.org/10.1007/s11120-009-9433-3

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  • DOI: https://doi.org/10.1007/s11120-009-9433-3

Keywords

  • Photosystem regulation
  • Aerobic photosynthetic bacteria
  • Bradyrhizobium

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