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Energetics of Photosystem II charge recombination in Acaryochloris marina studied by thermoluminescence and flash-induced chlorophyll fluorescence measurements

Photosynthesis Research volume 98, pages 131–140 (2008)Cite this article

Abstract

We studied the charge recombination characteristics of Photosystem II (PSII) redox components in whole cells of the chlorophyll (Chl) d-dominated cyanobacterium, Acaryochloris marina, by flash-induced chlorophyll fluorescence and thermoluminescence measurements. Flash-induced chlorophyll fluorescence decay was retarded in the μs and ms time ranges and accelerated in the s time range in Acaryochloris marina relative to that in the Chl a-containing cyanobacterium, Synechocystis PCC 6803. In the presence of 3-(3,4-dichlorophenyl)-1, 1-dimethylurea, which blocks the QB site, the relaxation of fluorescence decay arising from S2QA − recombination was somewhat faster in Acaryochloris marina than in Synechocystis PCC 6803. Thermoluminescence intensity of the so called B band, arising from the recombination of the S2QB − charge separated state, was enhanced significantly (2.5 fold) on the basis of equal amounts of PSII in Acaryochloris marina as compared with Synechocystis 6803. Our data show that the energetics of charge recombination is modified in Acaryochloris marina leading to a ~15 meV decrease of the free energy gap between the QA and QB acceptors. In addition, the total free energy gap between the ground state and the excited state of the reaction center chlorophyll is at least ~25–30 meV smaller in Acaryochloris marina, suggesting that the primary donor species cannot consist entirely of Chl a in Acaryochloris marina, and there is a contribution from Chl d as well.

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Fig. 1
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Scheme 1

Abbreviations

Chl:

Chlorophyll

DCMU:

3-(3,4-Dichlorophenyl)-1, 1-dimethylurea

Phe:

Pheophytin a

PQ:

Plastoquinone

PS:

Photosystem

QA and QB :

Primary and secondary electron acceptors in PSII

TL:

Thermoluminescence

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Acknowledgments

This study was supported from grants by the EU/Energy Network project SOLAR-H2 (FP7 contract 212508), and by the UK Biotechnology and Biological Research Council (BBSRC). The authors thank Dr. Min Chen for providing the Acaryochloris marina culture and the D1 and D2 protein sequences.

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Affiliations

  1. Institute of Plant Biology, Biological Resarch Center, 6726 Temesvari krt. 62, Szeged, Hungary

    Krisztián Cser, Zsuzsanna Deák & Imre Vass

  2. Division of Molecular Biosciences, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK

    Alison Telfer & James Barber

Authors
  1. Krisztián Cser
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  2. Zsuzsanna Deák
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  3. Alison Telfer
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  4. James Barber
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  5. Imre Vass
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Correspondence to Imre Vass.

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Cser, K., Deák, Z., Telfer, A. et al. Energetics of Photosystem II charge recombination in Acaryochloris marina studied by thermoluminescence and flash-induced chlorophyll fluorescence measurements. Photosynth Res 98, 131–140 (2008). https://doi.org/10.1007/s11120-008-9373-3

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  • DOI: https://doi.org/10.1007/s11120-008-9373-3

Keywords

  • Photosystem II
  • Charge separation
  • Electron transfer
  • Acaryochloris marina
  • Chlorophyll d
  • Thermoluminescence
  • Fluorescence