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Elucidating the site of action of oxalate in photosynthetic electron transport chain in spinach thylakoid membranes

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Abstract

The effects of oxalate on PS II and PS I photochemistry were studied. The results suggested that in chloride-deficient thylakoid membranes, oxalate inhibited activity of PS II as well as PS I. To our knowledge, this is the only anion so far known which inhibits both the photosystems. Measurements of fluorescence induction kinetics, Y •Z decay, and S2 state multiline EPR signal suggested that oxalate inhibited PS II at the donor side most likely on the oxygen evolving complex. Measurements of re-reduction of P700+ signal in isolated PS I particles in oxalate-treated samples suggested a binding site of oxalate on the donor, as well as the acceptor side of PS I.

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Abbreviations

DCPIP:

2,6-Dichlorophenolindophenol

DAD(red) :

Reduced 3,6-diaminodurene

F o :

Initial fluorescence, where all QA are oxidized

F v :

Variable fluorescence

MV:

Methyl viologen

TMPD(red) :

Reduced N,N,N 1,N 1-tetramethyl-p-phenylenediamine

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Acknowledgment

This work was supported by the Indo-Russian Joint project INT/ILTP/B-6.27 and by grants from the Russian Foundation for Basic Research.

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Authors and Affiliations

  1. School of Life Sciences, Devi Ahilya University, Vigyan Bhavan, Khandwa Road, Indore, 452017, M.P., India

    Anjana Jajoo, Archna Sahay, Pooja Singh, Sonal Mathur & Sudhakar Bharti

  2. Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia

    Anjana Jajoo, Sergei K. Zharmukhamedov, Vyacheslav V. Klimov, Suleyman I. Allakhverdiev & Sudhakar Bharti

Authors
  1. Anjana Jajoo
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  2. Archna Sahay
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  3. Pooja Singh
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  4. Sonal Mathur
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  5. Sergei K. Zharmukhamedov
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  6. Vyacheslav V. Klimov
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  7. Suleyman I. Allakhverdiev
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  8. Sudhakar Bharti
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Correspondence to Anjana Jajoo.

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Jajoo, A., Sahay, A., Singh, P. et al. Elucidating the site of action of oxalate in photosynthetic electron transport chain in spinach thylakoid membranes. Photosynth Res 97, 177–184 (2008). https://doi.org/10.1007/s11120-008-9314-1

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  • DOI: https://doi.org/10.1007/s11120-008-9314-1

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