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Spectral diffusion of the lowest exciton component in the core complex from Rhodopseudomonas palustris studied by single-molecule spectroscopy

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Abstract

We have recorded fluorescence-excitation spectra from individual RC–LH1 complexes from Rhodopseudomonas palustris. The spectra feature a few broad bands accompanied by a sharp line at the low-energy side of the spectrum which is ascribed to the lowest exciton state of the BChl a assembly. Recording several fluorescence-excitation spectra from the same individual complex in rapid succession reveals that the linewidth of the lowest exciton transition is determined by spectral diffusion which increases for higher excitation energies.

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Abbreviations

BChl a :

Bacteriochlorophyll a

H2Q:

Quinol

LH1:

Light-harvesting 1

LH2:

Light-harvesting 2

Q:

Quinone

Rb. :

Rhodobacter

RC:

Reaction centre

Rps. :

Rhodopseudomonas

Rs. :

Rhodospirillum

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Acknowledgments

RJC thanks the BBSRC for financial support.

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Authors and Affiliations

  1. Experimental Physics IV and Bayreuth Institute of Macromolecular Research (BIMF), University of Bayreuth, 95440, Bayreuth, Germany

    Martin F. Richter, Jürgen Baier, Silke Oellerich & Jürgen Köhler

  2. Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Biomedical Research Building, 120 University Place, Glasgow, G12 8TA, UK

    June Southall & Richard J. Cogdell

Authors
  1. Martin F. Richter
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  2. Jürgen Baier
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  3. June Southall
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  4. Richard J. Cogdell
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  5. Silke Oellerich
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  6. Jürgen Köhler
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Correspondence to Jürgen Köhler.

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Richter, M.F., Baier, J., Southall, J. et al. Spectral diffusion of the lowest exciton component in the core complex from Rhodopseudomonas palustris studied by single-molecule spectroscopy. Photosynth Res 95, 285–290 (2008). https://doi.org/10.1007/s11120-007-9267-9

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