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Growth enhancement of soybean (Glycine max) upon exclusion of UV-B and UV-B/A components of solar radiation: characterization of photosynthetic parameters in leaves

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An Erratum to this article was published on 12 December 2007

Abstract

Exclusion of UV (280–380 nm) radiation from the solar spectrum can be an important tool to assess the impact of ambient UV radiation on plant growth and performance of crop plants. The effect of exclusion of UV-B and UV-A from solar radiation on the growth and photosynthetic components in soybean (Glycine max) leaves were investigated. Exclusion of solar UV-B and UV-B/A radiation, enhanced the fresh weight, dry weight, leaf area as well as induced a dramatic increase in plant height, which reflected a net increase in biomass. Dry weight increase per unit leaf area was quite significant upon both UV-B and UV-B/A exclusion from the solar spectrum. However, no changes in chlorophyll a and b contents were observed by exclusion of solar UV radiation but the content of carotenoids was significantly (34–46%) lowered. Analysis of chlorophyll (Chl) fluorescence transient parameters of leaf segments suggested no change in the F v/F m value due to UV-B or UV-B/A exclusion. Only a small reduction in photo-oxidized signal I (P700+)/unit Chl was noted. Interestingly the total soluble protein content per unit leaf area increased by 18% in UV-B/A and 40% in UV-B excluded samples, suggesting a unique upregulation of biosynthesis and accumulation of biomass. Solar UV radiation thus seems to primarily affect the photomorphogenic regulatory system that leads to an enhanced growth of leaves and an enhanced rate of net photosynthesis in soybean, a crop plant of economic importance. The presence of ultra-violet components in sunlight seems to arrest carbon sequestration in plants.

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Abbreviations

Chl-a :

Chlorophyll a

DCMU:

3(3,4-Dicholorophenyl)-1,1-dimethyl urea

EDTA:

Ethylene diamine tetra acetic acid

EPR:

Electron paramagnetic resonance

F v :

Variable fluorescence of chlorophyll a

F m :

Maximum fluorescence of chlorophyll a

PAR:

Photosynthetically active radiation

PSI:

Photosystem I

PSII:

Photosystem II

QA :

Primary quinone acceptor of photosystem II

ROS:

Reactive oxygen species

UV-A:

Ultraviolet radiation of 320–400 nm

UV-B:

Ultraviolet radiation of 280–320 nm

UV-B/A:

Ultraviolet radiation of 280–400 nm

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Acknowledgments

PM thanks Indian National Science Academy (INSA), New Delhi for the award of Honorary Scientist grant for this study and Devi Ahilya University, Indore and Centre for Advanced Technology, Indore for providing the facilities. Financial supports by Council of Scientific and Industrial Research (CSIR) India to AT (9/301(109)/2K5-EMR-I) and SK (13(8050-A)-2005-Pool) are also thankfully acknowledged. The authors express thanks to Professor Govindjee for his visits to DAVV and delivering lectures on photosynthesis. This work was presented at the India-Russia bilateral ILTP meeting held on August 19, 2006 in Pushchino, Russia.

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Authors and Affiliations

  1. School of Life Sciences, DAVV, Vigyan Bhawan, Khandwa Road, Indore, 452001, India

    Guruprasad Kadur, Bhattacharjee Swapan, Kataria Sunita, Yadav Sanjeev, Tiwari Arjun, Baroniya Sanjay, Rajiv Abhinav & Prasanna Mohanty

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  1. Guruprasad Kadur
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  2. Bhattacharjee Swapan
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Correspondence to Prasanna Mohanty.

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An erratum to this article can be found at http://dx.doi.org/10.1007/s11120-007-9282-x

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Kadur, G., Swapan, B., Sunita, K. et al. Growth enhancement of soybean (Glycine max) upon exclusion of UV-B and UV-B/A components of solar radiation: characterization of photosynthetic parameters in leaves. Photosynth Res 94, 299–306 (2007). https://doi.org/10.1007/s11120-007-9190-0

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