Photosynthesis Research

, Volume 93, Issue 1–3, pp 27–43 | Cite as

The presence of multidomain linkers determines the bundle-shape structure of the phycobilisome of the cyanobacterium Gloeobacter violaceus PCC 7421

  • David W. Krogmann
  • Bertha Pérez-Gómez
  • Emma Berta Gutiérrez-Cirlos
  • Alicia Chagolla-López
  • Luis González de la Vara
  • Carlos Gómez-Lojero
Original paper


The complete genome sequence of Gloeobacter violaceus [Nakamura et al. (2003a, b) DNA Res 10:37–45, 181–201] allows us to understand better the structure of the phycobilisomes (PBS) of this cyanobacterium. Genomic analysis revealed peculiarities in these PBS: the presence of genes for two multidomain linker proteins, a core membrane linker with four repetitive sequences (REP domains), the absence of rod core linkers, two sets of phycocyanin (PC) α and β subunits, two copies of a rod PC associated linker (CpcC), and two rod cap associated linkers (CpcD). Also, there is one ferredoxin–NADP+ oxidoreductase with only two domains. The PBS proteins were investigated by gel electrophoresis, amino acid sequencing and peptide mass fingerprinting (PMF). The two unique multidomain linkers contain three REP domains with high similarity and these were found to be in tandem and were separated by dissimilar Arms. One of these, with a mass of 81 kDa, is found in heavy PBS fragments rich in PC. We propose that it links six PC hexamers in two parallel rows in the rods. The other unique linker has a mass of 91 kDa and is easily released from the heavy fragments of PBS. We propose that this links the rods to the core. The presence of these multidomain linkers could explain the bundle shaped rods of the PBS. The presence of 4 REP domains in the core membrane linker protein (129 kDa) was established by PMF. This core linker may hold together 16 AP trimers of the pentacylindrical core, or alternatively, a tetracylindrical core of the PBS of G. violaceus.


Cyanobacterium Gloeobacter violaceus Phycobilisome Multidomain linkers 





Gene encoding the α and β subunits of allophycocyanin


Gene encoding the 8–kDa core linker polypeptide


The 129-kDa core membrane linker phycobiliprotein


Absorbance units


Genes encoding the α and β subunits of phycocyanin


Gene encoding the PC-rod linker polypeptide


Gene encoding the 9-kDa rod linker polypeptide


Gene encoding the rod-core linker polypeptide






Ethylenediamine tetraacetic acid


Ferredoxin-NADP+ oxidoreductase


FNR containing only the two catalytic domains


FNR containing three domains (the CpcD-like domain and two catalytic domains)


Fluorescence units


Core linker polypeptide of 8 kDa


LR9, the 9-kDa, phycocyanin-associated rod linker polypeptide


CpcC, the PC-rod linker polypeptide


The rod core linker polypeptide


Polyacrylamide gel electrophoresis










Gene encoding FNR


Protein mass fingerprinting


Phenyl-methylsulfonyl fluoride


Repetitive sequence


Sodium dodecylsulfate


Whole-cell extract


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • David W. Krogmann
    • 1
  • Bertha Pérez-Gómez
    • 2
  • Emma Berta Gutiérrez-Cirlos
    • 3
  • Alicia Chagolla-López
    • 4
  • Luis González de la Vara
    • 4
  • Carlos Gómez-Lojero
    • 2
  1. 1.Department of BiochemistryPurdue UniversityWest LafayetteUSA
  2. 2.Departmento de BioquímicaCentro de Investigación y Estudios Avanzados-IPNMexicoMexico
  3. 3.Unidad de Biomedicina FES-Iztacala UNAMTlalnepantla, Edo de MéxicoMéxico
  4. 4.Cinvestav-Unidad IrapuatoGuanajuatoMexico

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