The Antenna System of Photosystem II From Thermosynechococcus elongatus at 3.2 Å Resolution

Abstract

The content and type of cofactors harboured in the Photosystem II core complex (PS IIcc) of the cyanobacterium Thermosynechococcus elongatus has been determined by biochemical and spectroscopic methods. 17 ± 1 chlorophyll a per pheophytin a and 0.25 β-carotene per chlorophyll a have been found in re-dissolved crystals of dimeric PS IIcc. The X-ray crystal structure of PS IIcc from Thermosynechococcus elongatus at 3.2 Å resolution clearly shows chlorophyll a molecules arranged in two layers close to the cytoplasmic and lumenal sides of the thylakoid membrane. Each of the cytoplasmic layers contains 9 chlorophyll a, whose positions and orientations are related by a local twofold rotation pseudo-C2 axis passing through the non-haem Fe2+. These chlorophyll a are arranged comparably to those in the antenna domains of PsaA and PsaB of cyanobacterial Photosystem I affirming an evolutionary relation. The chlorophyll a in the lumenal layer are less well conserved between Photosystems I and II and even between CP43 and CP47 with 4 chlorophyll a in the former and 7 in the latter.

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Abbreviations

Car:

β-carotene

cc:

core complex

Chla:

Chlorophyll a

cyt:

cytochrome

ETC:

electron transfer chain

LHC:

light harvesting complex

Pheoa:

Pheophytin a

PS:

Photosystem

QA:

primary quinone

QB:

secondary quinone

RPC:

reversed phase chromatography

T. elongatus :

Thermosynechococcus elongatus

TMH:

transmembrane α-helix

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Correspondence to Klaus-Dieter Irrgang.

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Loll, B., Kern, J., Zouni, A. et al. The Antenna System of Photosystem II From Thermosynechococcus elongatus at 3.2 Å Resolution. Photosynth Res 86, 175–184 (2005). https://doi.org/10.1007/s11120-005-4117-0

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Keywords

  • antenna system
  • crystal structure
  • excitation energy transfer
  • Photosystem II
  • Thermosynechococcus elongatus
  • the antenna system of Photosystem II