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Reaction pattern of Photosystem II: oxidative water cleavage and protein flexibility

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Abstract

This short communication addresses three topics of photosynthetic water cleavage in Photosystem II (PS II): (a) effect of protonation in the acidic range on the extent of the ‘fast’ ns kinetics of P680+· reduction by Y Z , (b) mechanism of O–O bond formation and (c) role of protein flexibility in the functional integrity of PS II. Based on measurements of light-induced absorption changes and quasielastic neutron scattering in combination with mechanistic considerations, evidence is presented for the protein acting as a functionally active constituent of the water cleavage machinery, in particular, for directed local proton transfer. A specific flexibility emerging above a threshold of about 230 K is an indispensable prerequisite for oxygen evolution and plastoquinol formation.

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Authors and Affiliations

  1. Max-Volmer-Laboratorium für Biophysikalische Chemie, Technische Universität Berlin, Straße des 17. Juni 135, 10623, Berlin, Germany

    Philipp Kühn, Hann-Jörg Eckert & Gernot Renger

  2. Hahn-Meitner-Institut, Glienicker Str. 100, 14109, Berlin, Germany

    Jörg Pieper & Ruep E. Lechner

  3. RAS, Institute of Basic Biological Problems, Pushchino, Moscow Region, Russia

    Olga Kaminskaya & Vladimir Shuvalov

Authors
  1. Philipp Kühn
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  2. Jörg Pieper
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  3. Olga Kaminskaya
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  4. Hann-Jörg Eckert
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  5. Ruep E. Lechner
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  6. Vladimir Shuvalov
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  7. Gernot Renger
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Correspondence to Gernot Renger.

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Kühn, P., Pieper, J., Kaminskaya, O. et al. Reaction pattern of Photosystem II: oxidative water cleavage and protein flexibility. Photosynth Res 84, 317–323 (2005). https://doi.org/10.1007/s11120-004-7079-8

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  • DOI: https://doi.org/10.1007/s11120-004-7079-8

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