The Chlamydomonas genome reveals its secrets: chaperone genes and the potential roles of their gene products in the chloroplast

Abstract

The first draft of the Chlamydomonas nuclear genome was searched for genes potentially encoding members of the five major chaperone families, Hsp100/Clp, Hsp90, Hsp70, Hsp60, the small heat shock proteins, and the Hsp70 and Cpn60 co-chaperones GrpE and Cpn10/20, respectively. This search yielded 34 potential (co-)chaperone genes, among them those 8 that have been reported earlier inChlamydomonas. These 34 genes encode all the (co-)chaperones that have been expected for the different compartments and organelles from genome searches in Arabidopsis, where 74 genes have been described to encode basically the same set of (co-)chaperones. Genome data from Arabidopsis and Chlamydomonas on the five major chaperone families are compared and discussed, with particular emphasis on chloroplast chaperones.

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Abbreviations

Cpn:

chaperonin

EST:

expressed sequence tag

HSP:

heat shock protein

ORF:

open reading frame

NBD:

nucleotide binding domain

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Correspondence to Michael Schroda.

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Schroda, M. The Chlamydomonas genome reveals its secrets: chaperone genes and the potential roles of their gene products in the chloroplast. Photosynth Res 82, 221–240 (2004). https://doi.org/10.1007/s11120-004-2216-y

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Keywords

  • chaperone
  • Chlamydomonas
  • chloroplast
  • Hsp100/Clp
  • Hsp60
  • Hsp70
  • Hsp90
  • sHsp