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Expression Profiling of the 56 R2R3-MYB Family Genes in Response to Cold, Drought, and Salt Stress in Blue Honeysuckle (Lonicera caerulea L.)

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Abstract

MYB transcription factors play a crucial role in plant stress responses, particularly in regulating abiotic stresses such as cold, drought, and salinity. In this study, we investigated the expression profiles of 56 MYB family genes in different tissues of blue honeysuckle (Lonicera caerulea L.) by qRT-PCR. Then, we analyzed the expression patterns of 56 MYB family genes under cold, drought, and salt stress treatments at different time points and examined their correlation with key stress-related genes. The results revealed that a total of 10, 4, 12, 3, 9, and 18 MYB genes were highly expressed in the roots, stems, leaves, flowers, buds, and fruits of blue honeysuckle, respectively. After cold stress treatment, 18 MYB genes showed a significantly positive correlation with CBF cold response pathway genes. After drought stress treatment, 4 MYB genes exhibited a notably positive correlation with drought stress key genes. After salt stress treatment, 34 MYB genes exhibited a notably positive correlation with salt stress key genes. These findings suggest a potential role of MYB family genes in responding to cold, drought, and salt stress in blue honeysuckle.

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Data Availability

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

This research was supported by the following funding: National Key R&D Program of China (2022YFD1600500); China Postdoctoral Science Foundation (2022MD713724); Natural Science Foundation of Heilongjiang Province, China (LH2020C008); Postdoctoral Fund of Heilongjiang Province, China (LBH-Z21117); China Agriculture Research System of MOF and MARA (CARS-29–10); and Central Government Supports the Reform and Development Fund Talent Training Project of Local Colleges and Universities, China.

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Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin, 150030, China

    Chunyang Bian, Haoyu Wang, Weihua Li, Jing Chen, Bingbing Ren, Dong Qin, Jiale Liu, Sitong Zang, Jiachen Li, Ke Ma, Junwei Huo & Huixin Gang

  2. National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, Northeast Agricultural University, Harbin, 150030, China

    Chunyang Bian, Haoyu Wang, Weihua Li, Jing Chen, Bingbing Ren, Dong Qin, Jiale Liu, Sitong Zang, Jiachen Li, Ke Ma, Junwei Huo & Huixin Gang

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  1. Chunyang Bian
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  2. Haoyu Wang
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  3. Weihua Li
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  11. Junwei Huo
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Contributions

G. and H. conceived and designed the experiments and reviewed and revised the manuscript. B., C., and R. conducted the experiments. W., Z., and L. prepared the plant material. B. and L. analyzed the data and wrote the manuscript with the help of Q.. G., M., and L. revised the manuscript and managed the project.

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Correspondence to Junwei Huo or Huixin Gang.

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Bian, C., Wang, H., Li, W. et al. Expression Profiling of the 56 R2R3-MYB Family Genes in Response to Cold, Drought, and Salt Stress in Blue Honeysuckle (Lonicera caerulea L.). Plant Mol Biol Rep (2024). https://doi.org/10.1007/s11105-024-01455-4

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