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Transcriptomic Profiling of Resistant and Susceptible Rice Cultivar Blast Resistance Genes During Magnaporthe oryzae Infection

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Abstract

Rice is an important staple food crop and a primary energy source for more than half of the world’s population. One of the deadly fungal diseases of rice produced by Magnaporthe oryzae is known as “blast disease.” It has consistently threatened the world’s food supply. This study aimed to dissect the complex blast resistance mechanism of the rice plant against blast pathogens. To understand the molecular mechanisms of blast resistance, we analyzed transcriptome changes in rice cultivars. Using transcriptome data obtained from an RNA-seq analysis of two samples comprising resistant (BR2655) and susceptible (HR12) genotypes under control and stress conditions was carried out. The total RNA sequencing generated a dataset of four samples, representing more than 40 Gb fastq raw data. A total of 145 million filtered reads were generated after the quality check. High-quality clean reads mapped rice reference genome RGAP 7. These aligned reads were taken for further analysis. A total of 11,867 DEGs across the genotypes under stress were identified. A total of 7577 and 4290 genes showed significant differential expression in BRC vs. BRIN library and HRC vs. HRIN library, respectively. The results reveal that differential gene expression analysis clearly shows the changes in the expression profiling of BR2655 and HR12 cultivars.

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Data will be made available upon reasonable request.

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Funding

The Indian Council of Medical Research funded the investigation via the sanction letter ICMR-SRF (IRIS ID NO. 2014–21680). The senior research fellow conducted this investigation and received a grant to support the design, gathering, analysis, and interpretation of data and produce the original study article.

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Authors and Affiliations

  1. Department of Molecular Biology, Yuvaraja’s College, University of Mysore, 570005, Mysuru, Karnataka, India

    R. Chandrakanth & N. S. Devaki

  2. Department of Plant Cell Biotechnology, CSIR-Central Food Technological Research Institute, Mysuru, 570020, India

    L. Sunil

Authors
  1. R. Chandrakanth
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  2. L. Sunil
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  3. N. S. Devaki
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Contributions

CR played a significant role in the conception, design of the work, and collection and interpretation of the data. SL evaluated, interpreted, and revision of the paper attentively. DNS interpretation, revised, and final approval of the article. The manuscript has been read and approved.

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Correspondence to R. Chandrakanth.

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Chandrakanth, R., Sunil, L. & Devaki, N.S. Transcriptomic Profiling of Resistant and Susceptible Rice Cultivar Blast Resistance Genes During Magnaporthe oryzae Infection. Plant Mol Biol Rep 42, 111–121 (2024). https://doi.org/10.1007/s11105-023-01404-7

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  • DOI: https://doi.org/10.1007/s11105-023-01404-7

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