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Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress

Abstract

Although sunflower (Helianthus annuus L.) is categorized as a medium drought-sensitive crop, in a changing climate scenario and/or with the onset of early droughts, the crop may be affected by water stress. This study characterizes and compares the gene expression profiles between aerial part and roots of two sunflower inbred lines with contrasting response to water stress: B59 (sensitive) and B71 (tolerant) under non-stress and water stress. Microarray analysis revealed that water stress induced significant changes in gene expression of both lines. The B59 line had a higher number of genes differentially expressed in water-stressed seedlings compared with B71 line. In both lines, most of the water stress responding genes was upregulated. In B59, the number of genes specifically upregulated in aerial part was higher than that observed in B71. In roots, B71 had more upregulated genes compared with B59. Genes involved in signaling pathway of hormones, components of redox system, and secondary metabolites were enriched in both organs of two lines. The knowledge generated could be helpful to provide tools, that together with the genetic engineering and molecular breeding, it would contribute for the development of water stress-tolerant varieties in crop plants.

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Acknowledgements

Dr. Julia Sabio y Garcia is gratefully acknowledged for critical reading of this manuscript.

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Correspondence to Sergio Alemano.

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Highlights

• Microarray revealed that water stress induced significant changes in gene expression of sunflower inbred lines.

• In B59, the genes upregulated in aerial part were higher than in B71. In roots, B71 had more upregulated genes compared with B59.

• Genes involved in signaling pathway of hormones, components of redox system, and secondary metabolites were enriched in both organs of two lines.

• The identification of unique genes may be used as candidate for selection of water stress tolerance traits

Electronic Supplementary Material

Supplementary Table 1

Description of differentially expressed genes (DEGs) in Aerial Part (AP) of B59 line in water stress vs. control contrast. (XLSX 404 kb)

Supplementary Table 2

Description of differentially expressed genes (DEGs) in Aerial Part (AP) of B71 line in water stress vs. control contrast. (XLSX 281 kb)

Supplementary Table 3

Description of differentially expressed genes (DEGs) in Roots (R) of B59 line in water stress vs. control contrast. (XLSX 216 kb)

Supplementary Table 4

Description of differentially expressed genes (DEGs) in Roots (R) of B71 line in water stress vs. control contrast. (XLSX 262 kb)

Supplementary Table 5

Primer sequences for gene expression analysis derived from RT -qPCR analysis. (30 kb)

Supplementary Table 6

Description of unique differentially expressed genes (DEGs) up-regulated in Aerial Part (AP) of B59 line in water stress vs. control contrast. (XLSX 56 kb)

Supplementary Table 7

Description of unique differentially expressed genes (DEGs) down-regulated in Aerial Part (AP) of B59 line in water stress vs. control contrast. (XLSX 62 kb)

Supplementary Table 8

Description of unique differentially expressed genes (DEGs) up-regulated in Aerial Part (AP) of B71 line in water stress vs. control contrast. (XLSX 36 kb)

Supplementary Table 9

Description of unique differentially expressed genes (DEGs) down-regulated in Aerial Part (AP) of B71 line in water stress vs. control contrast. (XLSX 31 kb)

Supplementary Table 10

Description of unique differentially expressed genes (DEGs) up-regulated in Roots (R) of B59 line in water stress vs. control contrast. (XLSX 22 kb)

Supplementary Table 11

Description of unique differentially expressed genes (DEGs) down-regulated in Roots (R) of B59 line in water stress vs. control contrast. (XLSX 26 kb)

Supplementary Table 12

Description of unique differentially expressed genes (DEGs) up-regulated in Roots (R) of B71 line in water stress vs. control contrast. (XLSX 35 kb)

Supplementary Table 13

Description of unique differentially expressed genes (DEGs) down-regulated in Roots (R) of B71 line in water stress vs. control contrast. (XLSX 39 kb)

Supplementary Figure 1

Validation of microarrays of parental lines B71 and B59 under conditions of water stress. Log2 (FC) of the ten selected genes. Elongation factor (Ha.EF1α) and α-TUB were used as reference genes. (JPG 786 kb)

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Escalante, M., Vigliocco, A., Moschen, S. et al. Transcriptomic Analysis Reveals a Differential Gene Expression Profile Between Two Sunflower Inbred Lines with Different Ability to Tolerate Water Stress. Plant Mol Biol Rep 38, 222–237 (2020). https://doi.org/10.1007/s11105-020-01192-4

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  • DOI: https://doi.org/10.1007/s11105-020-01192-4

Keywords

  • Sunflower
  • Water stress
  • Gene expression profile
  • Microarray
  • Aerial part
  • Root