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OsSGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Phenolics in Rice

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Abstract

Phenolics are a class of plant secondary metabolites that play important roles in plant growth and environmental adaptation. Glucosylation of phenolics is one of the molecular mechanisms controlling phenolics homeostasis. However, the relevant glucosyltransferases are largely unknown. In this study, a putative family 1 glucosyltransferase gene OsSGT1 was cloned from rice due to its close homology with the previously reported phenolics-related glucosyltransferases UGT84A1-A4, and the phylogenetic relationship of OsSGT1 with homologs from other species was investigated. Recombinant OsSGT1 protein showed strong activity towards phenolics to form their glucose conjugates. This is the first identified natural phenolics-related glucosyltransferase in rice. In addition, the expression patterns of OsSGT1 in different tissues of rice indicated that OsSGT1 was predominantly expressed in the old leaves and dough grains, suggesting that OsSGT1 might be involved in the maturation process of rice by regulating phenolic metabolism, and thus deepened our understanding on the roles of phenolics in rice growth and environmental adaptation.

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Contributions

BKH and QL conceived and designed the research. QL, YYZ, and LC conducted the experiments. TTC and YJL contributed analytical tools and analyzed data. QL, TTC, and BKH wrote the manuscript. All authors read and approved the manuscript.

Funding

This study was financially supported by key R & D project of Shandong Province of China (no. 2018GNC110019).

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Authors and Affiliations

  1. The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education; School of Life Science, Shandong University, Qingdao, 266237, China

    Qian Liu, Yu-ying Zhang, Lu Chen, Ting-ting Chen, Yan-jie Li & Bing-kai Hou

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  1. Qian Liu
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  2. Yu-ying Zhang
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  3. Lu Chen
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  4. Ting-ting Chen
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  5. Yan-jie Li
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  6. Bing-kai Hou
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Correspondence to Bing-kai Hou.

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Fig S1

HPLC analysis of reaction mixtures of different phenolics with heat-inactivated OsSGT1 as negative control. (a-e) denote sinapic acid, ferulic acid, caffeic acid, cinnamic acid and coumaric acid, respectively; 1 denotes the reactions with inactivated GST-OsSGT1. 2 denotes the authentic standards of phenolics. (DOCX 157 kb)

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Liu, Q., Zhang, Yy., Chen, L. et al. OsSGT1 Is a Glucosyltransferase Gene Involved in the Glucose Conjugation of Phenolics in Rice. Plant Mol Biol Rep 37, 41–49 (2019). https://doi.org/10.1007/s11105-019-1134-2

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