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Genome Wide Identification of C2H2-Type Zinc Finger Proteins of Tomato and Expression Analysis Under Different Abiotic Stresses

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Abstract

In plants, C2H2-type zinc finger proteins play important roles in multiple processes, including plant growth and development, as well as biotic and abiotic responses. In the present study, based on the presence of the C2H2 domain (CX2~4CX3FX5LX2HX3~5H), 112 C2H2-type zinc finger proteins were predicted in tomato. Through gene and protein structures analyses and phylogenetic analysis, the 112 C2H2-type zinc finger proteins were divided into five subfamilies. Members of the same subfamily shared similarities in gene and protein structures, while members of different subfamilies contained different numbers of the C2H2 domain. The tissue expression pattern analysis showed that 24 C2H2-type zinc finger proteins are constitutively expressed in all tissues, indicating that they may play important roles in the growth and development of all tissues. In addition, under chilling (4 °C), heat (42 °C), high salinity (200 Mm NaCl), and osmotic (20% PEG) stresses, members of C2H2-type zinc finger family were induced to varying degrees, which suggested that these genes were involved in multiple abiotic stress responses. This study will provide theoretical basis for further research of C2H2-type zinc finger proteins in tomato.

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Abbreviations

PEG:

Polyethylene glycol

TFIIIA:

Transcription factor IIIA

SUF4:

SUPPRESSOR OF FRIGIDA 4

KNU:

KNUCKLES

GIS:

GLABROUS INFLORESCENCE STEMS

EAR:

ERF-associated amphiphilic repression

SlC2H2:

Solanum lycopersicum C2H2

TSS:

Transcription start site

MS:

Murashige and Skoog

SA:

Salicylic acid

GA:

Gibberellins

MeJA:

Methyl jasmonate

ET:

Ethylene

ABA:

Abscisic acid

IAA:

Indole acetic acid

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Funding

This work was supported the National Natural Science Foundation of China (grant number 31870277) and Funds of the Shangdong ‘Double Tops’ Program.

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Author notes

  1. Nan Ming and Nana Ma contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai’an, 271018, Shandong, China

    Nan Ming, Nana Ma, Baozhen Jiao, Wei Lv & Qingwei Meng

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  1. Nan Ming
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  2. Nana Ma
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  3. Baozhen Jiao
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  4. Wei Lv
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  5. Qingwei Meng
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Correspondence to Wei Lv or Qingwei Meng.

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Key Message

• One hundred twelve C2H2 proteins were predicted in tomato and divided into five subfamilies. The tissue expression profile and abiotic stress induction expression pattern implied that tomato C2H2 proteins were involved in multiple abiotic stress responses and might play a role in tomato growth.

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Ming, N., Ma, N., Jiao, B. et al. Genome Wide Identification of C2H2-Type Zinc Finger Proteins of Tomato and Expression Analysis Under Different Abiotic Stresses. Plant Mol Biol Rep 38, 75–94 (2020). https://doi.org/10.1007/s11105-019-01182-1

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