Expression Analysis of TCP Genes in Peach Reveals an Involvement of PpTCP.A2 in Ethylene Biosynthesis During Fruit Ripening

Abstract

Plant-specific TCP transcription factor has been studied in plant growth and development, but little is known the roles during fruit development and ripening. Based on phylogenetic analysis, in this study, the 20 TCP genes isolated from peach were divided into two classes (I and II) comprised by four groups (A → D), and distributed into 19 clusters. Expression profiles of TCP genes in peach were analyzed to evaluate their potential roles during fruit development and ripening. The result showed that there are four genes (PpTCP.A2, PpTCP.A10, PpTCP.B1, and PpTCP.C1) and each gene had similar expression patterns detected by qRT-PCR to that tested by RNA-Seq, and higher expressed in fruitlets than in other developmental and ripening fruits, suggesting that these four genes were probably related to early development in peach fruit. Noteworthy, PpTCP.A2 was lower expressed in ripening fruits than in developmental or pre-ripening fruits of 11 peach cultivars, and virus-induced silencing PpTCP.A2 increased ethylene production and promoted PpACS1, but not PpACO1 and PpendoPGM expression. This result indicates that PpTCP.A2 was a transcription repressor, which could regulate ethylene biosynthesis by affecting PpACS1 expression, and thus should be involved in fruit ripening.

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Abbreviations

TCP:

TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL

bHLH:

Basic Helix-Loop-Helix

TB:

TEOSINTE BRANCHED

CYC:

CYCLOIDEA

PCF:

PROLIFERATING CELL FACTOR

CIN:

CINCINNATA

Pp :

Prunus persica

Fv :

Fragaria × ananassa

Sl :

Solanum lycopersicon

ACS:

1-Aminocyclopropane-1-carboxyla synthase

ACO:

1-Aminocyclopropane-1-carboxyla oxidase

EndoPG3:

Endopolygalacturonase-mediating melting flesh phenotype

RIN:

RIPENING INHIBITOR

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Funding

The work was supported by the National Natural Science Foundations of China (31471856 and 31672118), the Natural Science Foundation of Jiangsu Province (BK20171380), and the Fundamental Research Funds for the Central Universities (KYZ201732).

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Correspondence to Chao Gu.

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Guo, ZH., Shu, WS., Cheng, HY. et al. Expression Analysis of TCP Genes in Peach Reveals an Involvement of PpTCP.A2 in Ethylene Biosynthesis During Fruit Ripening. Plant Mol Biol Rep 36, 588–595 (2018). https://doi.org/10.1007/s11105-018-1105-z

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Keywords

  • Peach
  • TCP transcription factor
  • Fruit ripening
  • Virus-induced gene silencing
  • Phylogenetic classification