Silicon Coordinates DNA Replication with Transcription of the Replisome Factors in Diatom Algae

Original Paper

Abstract

Transcription is a key in coordinating the action of actors involved in DNA replication. The present research characterizes the impact of silicon on transcription for selected replicative genes in two diatom microalgae: Thalassiosira pseudonana and Phaeodactylum tricornutum. We show a relationship between the RNA expression of major replicative actors and silicon concentration using q-PCR and SSH. Thanks to phylogenetical homologies with diatoms and eukaryotic genes, these results could indicate a new regulation process of cell cycle for all living beings. Our results tend to confirm initially the central role of mRNA expression control in regulating DNA replication. Silicon, as an epigenomic element, seems to impact on the expression of 15 target genes belonging to the following groups: the polymerases group, POLA2 p68 and PRIM1 p49 (DNA polymerase α/primase), DNA polymerase ε POLE1, DNA polymerase δ POLD, the PCNA sliding clamp, the ternary complex (CMG group, CDC45, subunit PSF2 of GINS, helicase subunit MCM 2,3,6), and the “maturation-promoting factor” (MPF group, CDK-A (p34cdc2). Finally, we demonstrate, by comparing the two models, that silicon promotes the expression of POLD1, POLE1, and POLE3, and PSF2 mRNA expression.

Keywords

Cell cycle Silicon Epigenetic Replisome Transcription level Diatom microalgae 

Abbreviations

2-ODDs

2-Oxoacid-dependent dioxygenase

cDNA

Complementary DNA

CR

Crossing point

MPF

Mitosis promoter factor

ORC

Origin recognition complex

PRC

Pre-replication complex

P. tricornutum

Phaeodactylum tricornutum

RNAi

RNA interference

RPA

Replication protein A

RO

Replication origins

RT-qPCR

Quantitative reverse transcription PCR

silicon

Silicic acid

SPF

S-phase-promoting factors

SSH

Suppressive subtractive hybridization

T. pseudonana

Thalassiosira pseudonana

Notes

Acknowledgements

We are grateful to JGI institute for providing access to T. pseudonana genomes. We thank Dr. Chris Bowler from E.N.S. for providing us the cell strain of Phaeodactylum tricornutum, and we also thank him for associating us to the Phaeodactylum tricornutum genotyping team. We thank the cell biology department of M.M.S. laboratory from the University of Nantes, for offering us a technical assistance.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interests.

Supplementary material

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Supplementary Table 1

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Mer Molécule Sante (MMS) Institute, Department of Cell & Molecular BiologyUniversity of SciencesNantesFrance
  2. 2.IICiMed EA 1155University of SciencesNantesFrance

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