Lectin Protein Kinase Is Induced in Plant Roots in Response to the Endophytic Fungus, Piriformospora indica
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The symbiotic association of Piriformospora indica provides growth promotion leading to increased biomass as well as enhanced plant tolerance to biotic and abiotic stresses in a variety of plant species. Since various protein kinases are thought to be involved in mutual signaling in symbiotic interactions, we analyzed transcript profile of rice protein kinase genes during the infection process by real-time PCR. A rice L-type lectin protein kinase (OslecRK) and Arabidopsis AtLecRK genes are highly upregulated in roots during the initial stage of symbiotic association. In order to investigate the role of LecRK gene in root colonization, the Arabidopsis mutant line (Atlecrk) of rice homolog was used which shows the restricted intracellular penetration of the P. indica hyphae. The results indicate that LecRK might be a crucial factor in perception and recognition during plant–P. indica association by regulating fungal invasion.
KeywordsPiriformospora indica Lectin protein kinase Rice Real-time PCR Symbiotic association
The work was partially supported by the Department of Biotechnology (DBT), Government of India, and the National Institute for Plant Genome Research, New Delhi. Nivedita gratefully acknowledges a research fellowship from the Council of Scientific and Industrial Research (CSIR), Government of India.
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