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Isolation and Characterization of Microsatellite Markers for Viola websteri (Violaceae) and Cross-Species Amplification within the Genus Viola

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Abstract

We isolated and characterized microsatellite loci in Viola websteri (Violaceae), an endangered species from Korea and endemic to Northeast Asia. A total of 27 microsatellite loci were developed and tested in Korean and Chinese populations. The number of alleles per locus varied from two to eight. The observed and expected heterozygosities within two populations were 0.000 to 1.000 and 0.080 to 0.816, respectively. Korean and Chinese populations were clearly distinguished by the private alleles from 16 loci. A total of 21 loci out of the 27 developed loci were successfully cross-amplified in 39 other Viola species. We believe that these microsatellite loci will be useful for future studies on genetic diversity and population structure of V. websteri, as well as other Viola species.

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Acknowledgments

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR201503102) under a memorandum of understanding on cooperation for biological research between NIBR and Institute of Botany of the Chinese Academy of Sciences, People’s Republic of China. The authors thank Changgun Lim, Hyosig Won, and Jina Lim for their help collecting the samples and the two anonymous reviewers for their valuable comments.

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Authors and Affiliations

  1. Plant Resources Division, National Institute of Biological Resources, Incheon, 404-708, Korea

    Jong-Soo Kang & Myounghai Kwak

  2. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China

    Xianchun Zhang

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  1. Jong-Soo Kang
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  2. Xianchun Zhang
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Correspondence to Myounghai Kwak.

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ESM 1

The fragment sizes of the distinguishable loci in both populations. (XLSX 12 kb)

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Kang, JS., Zhang, X. & Kwak, M. Isolation and Characterization of Microsatellite Markers for Viola websteri (Violaceae) and Cross-Species Amplification within the Genus Viola . Plant Mol Biol Rep 35, 145–153 (2017). https://doi.org/10.1007/s11105-016-1011-1

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