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Phosphoproteomic Analysis in Phaseolus vulgaris Roots Treated with Rhizobium etli Nodulation Factors

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Abstract

Legumes are unique in their ability to establish symbiotic interactions with rhizobacteria, providing a source of assimilable nitrogen; this symbiosis is regulated by complex signaling process between the plant and the bacteria. The participation of specific protein kinases during the initial steps of the nodulation process has been established. However, their substrates or the signaling networks implicated are not fully understood. Herein, a phosphoproteomic analysis of Phaseolus vulgaris roots treated for 24 h with specific Nod factors was performed using an immobilized metal ion affinity chromatography enrichment and two-dimensional gel electrophoresis approach with mass spectrometry identification. A total of 33 protein spots showing more than 1.5-fold shift were identified (17 protein spots in which the relative abundance increased and 16 that decreased). The majority of the identified root phosphoproteins displaying an increased relative abundance are presumed to have functions related to the biosynthesis and folding of proteins, energy metabolism, or cytoskeleton rearrangements, which reflect the metabolic status of the roots as being part of the developmental processes leading to nodule initiation and the importance of cytoskeleton rearrangement in the P. vulgaris–rhizobia symbiosis. The proteins in which relative abundance decreased are associated with defense and oxido-reduction processes, which could indicate a suppression of plant defense responses during the establishment of the rhizobia–legume interaction and an increase of reactive oxygen species production.

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Acknowledgments

This work was supported by Dirección General de Asuntos del Personal Académico – Universidad Nacional Autónoma de México (IN201312). We thank Drs. Federico Sánchez and Rosana Sánchez-Lopez for critical reading of the manuscript and Olivia Santana for the purification of R. etli Nod factors.

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Authors and Affiliations

  1. Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos, 62210, México

    David Jáuregui-Zúñiga, Yolanda Ortega-Ortega & Carmen Quinto

  2. Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, UNAM, Apartado Postal 510-3, Cuernavaca, Morelos, 62210, México

    Martha Pedraza-Escalona

  3. Instituto Nacional de Medicina Genómica, México, D.F., 14610, México

    Juan Pablo Reyes-Grajeda

  4. IFaB SA de CV, México, D.F., 14610, México

    María Isabel Ruiz

Authors
  1. David Jáuregui-Zúñiga
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  2. Yolanda Ortega-Ortega
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  3. Martha Pedraza-Escalona
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  5. María Isabel Ruiz
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  6. Carmen Quinto
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Correspondence to Carmen Quinto.

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Jáuregui-Zúñiga, D., Ortega-Ortega, Y., Pedraza-Escalona, M. et al. Phosphoproteomic Analysis in Phaseolus vulgaris Roots Treated with Rhizobium etli Nodulation Factors. Plant Mol Biol Rep 34, 961–969 (2016). https://doi.org/10.1007/s11105-016-0978-y

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  • DOI: https://doi.org/10.1007/s11105-016-0978-y

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