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Gibberellins Affect Tobacco Growth and Development in a Dose Dependent Manner through Genes Involved in Metabolic Processes

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Summary

Gibberellins (GAs) play an important role in growth control in plants, but little is known regarding their effects on metabolic processes, which can further regulate plant growth. To increase our understanding of the metabolic regulatory mechanism of GAs during plant growth and development, microarray analysis was performed to identify a subgroup of GA-regulated genes by comparing the expression levels of genes in GA over-accumulation, wild-type (WT), and GA downregulated tobacco plants. The modulation of gene expression and metabolic adjustment in response to changes in plant growth in the presence of various GA concentrations was evaluated. The results showed that increasing endogenous bioactive GA levels increased leaf growth, with giant phenotypes were observed in the presence of bioactive GA levels in the three tobacco lines. Moreover, GA affects mainly the early period of plant growth rather than the later stages. Microarray analyses indicated that 163 genes (FC > 3.0) were affected by the GA concentration. Three key genes related to lipid metabolism, one gene involved in nitrogen metabolism, and four genes involved in cell wall component synthesis were regulated by GA. These results revealed an interaction between GAs and growth control, which coordinates lipid metabolism, nitrogen metabolism, and cell wall extension. The findings increase our understanding on the role of GAs in plant growth and development at the level of gene expression.

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Acknowledgment

This work was supported by grants from The Fundamental Research Funds for the Central Universities (No. YX2014-12), Program for Changjiang Scholars and Innovative Research Team in University (IRT13047), and the State Forestry Bureau 948 Project (No. 2012-4-40).

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Authors and Affiliations

  1. National Engineering Laboratory for Forest Tree Breeding, Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Zhexin Li, Shihui Niu, Huwei Yuan & Wei Li

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  1. Zhexin Li
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  2. Shihui Niu
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  4. Wei Li
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Correspondence to Wei Li.

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Li, Z., Niu, S., Yuan, H. et al. Gibberellins Affect Tobacco Growth and Development in a Dose Dependent Manner through Genes Involved in Metabolic Processes. Plant Mol Biol Rep 33, 1259–1269 (2015). https://doi.org/10.1007/s11105-014-0832-z

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