Pea p68 Imparts Salinity Stress Tolerance in Rice by Scavenging of ROS-Mediated H2O2 and Interacts with Argonaute
- 435 Downloads
The p68, a prototypic member of DEAD-box protein family, is involved in pre-mRNA splicing, RNA-induced silencing and transcription regulation. However, the role of plant p68 in stress tolerance and molecular targets responsible for this has not been reported. Here, we report the isolation and characterization of salinity-induced pea p68 (Psp68). The expression of Psp68 was more in aerial parts as compared to the roots in response to the abiotic stress. The transgenic-overexpressing Psp68 showed enhanced tolerance to salinity stress by efficiently attenuating ionic adjustment and scavenging reactive oxygen species (ROS). The enhanced tolerance was further confirmed by observing several physiological indices. Psp68-overexpressing rice accumulated higher K+ and Ca2+ and lower Na+ level as compared to the wild-type (WT) plants. Furthermore, Psp68 interacts with pea argonaute (AGO1), a catalytic component of the RNA-induced silencing complex (RISC) responsible for the gene silencing. The microarray analysis showed that Psp68 regulates many transcripts involved in the abiotic and oxidative stress responses as well as gene-silencing mechanisms in rice. Thus, the Psp68 functions as a molecular switch in different signaling pathways leading to stress tolerance. Overall, Psp68 may serve as a useful biotechnological tool for the improvement of stress tolerance crops.
KeywordsAbiotic stress Antioxidant Argonaute Microarray analysis Oryza sativa Pea p68 ROS Salinity stress
The authors gratefully acknowledge ICGEB for awarding “The Arturo Falaschi ICGEB Pre-doctoral Fellowship” to M.S.A.B. and K.M.K.H. Work on plant helicases and abiotic stress tolerance in NT’s laboratory is fully supported by the Department of Biotechnology (DBT) and partially by ICGEB, New Delhi. We do not have any conflict of interest to declare.
- Amin M, Elias SM, Hossain A, Ferdousi A, Rahman MS, Tuteja N, Seraj ZI (2012) Overexpression of a DEAD box helicase, PDH45, confers both seedling and reproductive stage salinity tolerance to rice (Oryza sativa L.). Mol Breed 30:345–354Google Scholar
- Bhattacharjee S (2012) The language of reactive oxygen species signaling in plants. J Bot 1–22. doi: 10.1155/2012/985298
- Dhanda SS, Sethi GS (1998) Inheritance of excised-leaf water loss and relative water content in bread wheat (Triticum aestivum). Euphytica 104:39–47Google Scholar
- Garg B, Jaiswal JP, Misra S, Tripathi BN, Prasad M (2012) A comprehensive study on dehydration-induced antioxidative responses during germination of Indian bread wheat (Triticum aestivum L. em Thell) cultivars collected from different agroclimatic zones. Physiol Mol Biol Plants 18:217–228PubMedCentralPubMedGoogle Scholar
- Gill SS, Singh LP, Gill R, Tuteja N (2012) Generation and scavenging of reactive oxygen species in plants under stress. In: Tuteja N, Gill SS, Tiburcio AF, Tuteja R (eds) Improving crop resistance to abiotic stress. Wiley-VCH Verlag, Germany, pp 49–70Google Scholar
- Hughes FM Jr, Cidlowski JA (1999) Potassium is a critical regulator of apoptotic enzymes in vitro and in vivo. Adv Enzym Regul 39:157–171Google Scholar
- Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Report 5:387–405Google Scholar
- Kaur N, Gupta AK (2005) Signal transduction pathways under abiotic stresses in plants. Curr Sci 88:1771–1780Google Scholar
- Quintero FJ, Martinez-Atienza J, Villalta I, Jiang X, Kim WY, Ali Z, Fujii H, Mendoza I, Yun DJ, Zhu JK, Pardo JM (2011) Activation of the plasma membrane Na/H antiporter salt-overly-sensitive 1 (SOS1) by phosphorylation of an auto-inhibitory C-terminal domain. Proc Natl Acad Sci U S A 108:2611–2616PubMedCentralPubMedGoogle Scholar
- Zhi, Palladina TO (2011) Function of transport H+-ATPases in plant cell plasma and vacuolar membranes of maize under salt stress conditions and effect of adaptogenic preparations. Ukr Biokhim Zh 83:63–68Google Scholar