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Characterization of Transcription Factors Following Expression Profiling of Medicago truncatula–Botrytis spp. Interactions

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Abstract

Medicago truncatula may be used as a model plant to study the pathosystem Vicia faba/Botrytis spp. This study aims to investigate the profiling of the transcription factors (TFs) of M. truncatula involved in the interaction with Botrytis cinerea and Botrytis fabae. The response of two genotypes of M. truncatula to the pathogens was first established, defining genotype A17 as partially resistant and genotype Esp162 as susceptible. We found that B. fabae is more aggressive as a pathogen of M. truncatula than B. cinerea. The profiling of the TFs involved in the interaction was subsequently carried out using an available genome guide qPCR-based platform for the quantitative measurement of 1,084 M. truncatula TFs. Microscopic studies showed that the infection process had started 24 h after inoculation. A total of 126 TFs showed significant differences in their expression after inoculation irrespective of the genotype, while 37 TFs were differentially expressed between the resistant and the susceptible genotypes and 70 TFs showed different levels of expression between the genotypes unaltered by infection. This has allowed characterization at the transcriptional regulation level of the differential response of two genotypes of M. truncatula to two very similar pathogens differing in their aggressiveness. These results will be valuable to increase the functional knowledge of the M. truncatula genome and to help in breeding programmes of V. faba for resistance to Botrytis spp.

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Authors and Affiliations

  1. Institute for Sustainable Agriculture, CSIC, Apdo. 4084, 14080, Córdoba, Spain

    Ángel M. Villegas-Fernández, Eva Madrid & Diego Rubiales

  2. Max Planck Institute of Molecular Plant Physiology, Science Park, 14476, Postdam-Golm, Germany

    Franziska Krajinski & Armin Schlereth

Authors
  1. Ángel M. Villegas-Fernández
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  2. Franziska Krajinski
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  3. Armin Schlereth
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  4. Eva Madrid
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  5. Diego Rubiales
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Correspondence to Ángel M. Villegas-Fernández.

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Villegas-Fernández, Á.M., Krajinski, F., Schlereth, A. et al. Characterization of Transcription Factors Following Expression Profiling of Medicago truncatula–Botrytis spp. Interactions. Plant Mol Biol Rep 32, 1030–1040 (2014). https://doi.org/10.1007/s11105-014-0710-8

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