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The SCF E3 Ligase AtPP2-B11 Plays a Negative Role in Response to Drought Stress in Arabidopsis

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Abstract

Ubiquitination is a eukaryotic post-translational protein modification, implicated in various cellular processes in higher plants, and E3 ligases play an essential role in this cascade. In this study, we identified an Skp1–Cullin–F-box (SCF) E3 ligase encoding gene, Arabidopsis phloem protein 2-B11 (AtPP2-B11; At1g80110), by searching drought-responsive element in the promoter regions of 1,488 putative E3s in the Arabidopsis genome. The expression of AtPP2-B11 in seedlings was remarkably induced with increased duration of drought treatment. Transgenic lines overexpressing AtPP2-B11 exhibited obvious hypersensitivity to drought stress during seed germination and in mature plants. Protein interaction assay with Arabidopsis Skp1 (ASK) proteins showed that AtPP2-B11 strongly interacted with ASK 7, ASK 18, and ASK 19, respectively, indicating that AtPP2-B11 may function as an F-box protein. An AtPP2-B11-interacting protein, AtLEA14, was identified by a yeast two-hybrid screening and bimolecular fluorescence complementation assay. Real-time quantitative RT-PCR and Western blot analysis showed that the transcript and protein levels of AtLEA14 decreased in 35S::AtPP2-B11 transgenic plants subjected to drought conditions. Transgenic Arabidopsis plants overexpressing AtPP2-B11 showed altered expression of abiotic stress response marker genes (COR15a, COR47, ERD10, KIN1, RAB18, and RD22) under both normal and drought conditions. Overall, these results suggested that AtPP2-B11, a SCF E3 ligase, played an important role in response to drought stress as a negative regulator in Arabidopsis.

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Acknowledgments

This work was supported by the National Basic Research Program (grant no. 2012CB114200), the National Natural Science Foundation (grant no. 31000121 and 30970225) in China.

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  1. Yanze Li

    Present address: National Engineering Research Center for Beijing Biochip Technology, 18 Life Science Parkway, Changping District, Beijing, 102206, People’s Republic of China

Authors and Affiliations

  1. State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, People’s Republic of China

    Yanze Li, Fengjuan Jia, Lu Luo, Jinguang Huang, Guodong Yang, Changai Wu & Chengchao Zheng

  2. Maize Institute, Shandong Academy of Agricultural Sciences/National Maize Improvement Sub-Center, Jinan, 250100, People’s Republic of China

    Yanli Yu

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Yanze Li and Fengjuan Jia contributed equally to this study.

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Fig. S1

Sequence alignments of AtPP2-B11 and other known F-box proteins (DOC 58 kb)

Table S1

Primers for semiquantitative RT-PCR or real-time quantitative RT-PCR using in this study (DOC 54 kb)

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Li, Y., Jia, F., Yu, Y. et al. The SCF E3 Ligase AtPP2-B11 Plays a Negative Role in Response to Drought Stress in Arabidopsis . Plant Mol Biol Rep 32, 943–956 (2014). https://doi.org/10.1007/s11105-014-0705-5

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