Abstract
A homology-based cloning strategy yielded two cDNA clones presumably encoding Rac/Rop GTPases, Am-rac1 and Am-rac2, from callus cultures of Aquilaria microcarpa. Transcriptional levels of Am-rac1, measured using reverse transcription polymerase chain reaction, remained steady, while those of Am-rac2 increased dramatically following treatment of cultured cells with either a yeast extract or methyl jasmonate. These results suggested that Am-rac1 was a constitutively expressed homologue, while Am-rac2 was induced in A. microcarpa cells. Moreover, a farnesyl diphosphate synthase gene of A. microcarpa, Am-FaPS1, was transcriptionally activated in A. microcarpa cells grown in the presence of methyl jasmonate; however, expression levels were markedly lowered in the presence of various signal transduction-related inhibitors involved in Ca2+-, Rap/Rop GTPase-, or ubiquitin-dependent signaling processes. Whereas, expression of Am-FaPS1 was markedly increased, even in the absence of methyl jasmonate in A. microcarpa cells over-expressing Am-rac2. These findings suggested that Rac/Rop GTPase proteins played important roles in jasmonate-induced enhancement of terpenoid metabolism in A. microcarpa.
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Abbreviations
- CAM:
-
Calmodulin
- FaPS:
-
Farnesyl diphosphate synthase
- MJ:
-
Methyl jasmonate
- RACE:
-
Rapid amplification of cDNA end
- RT:
-
Reverse transcription
References
Berken A (2006) Rho GTPases in plants—molecular mechanisms and signaling pathways in the process of fertilization. Cell Mol Life Sci 63:2446–2459
Creelman RA, Mullet ME (1997) Biosynthesis and action of jasmonates in plants. Ann Rev Plant Physiol Plant Mol Biol 48:355–381
Gao Y, Dickerson JB, Guo F, Zheng J, Zheng Y (2004) Rational design and characterization of a Rac GTPase-specific small molecule inhibitor. Proc Nat Acad Sci USA 101:7618–7623
Gu Y, Wang Z, Yang Z (2004) ROP/RAC GTPase: an old new master regulator for plant signaling. Curr Opin Plant Biol 7:527–536
Gundlach H, Muller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Pro Nat Acad Sci USA 89:2389–2393
Ito M, Honda G (2005) Taxonomical identification of agarwood-producing species. Nat Med 59:104–112
Ivanchenko M, Vejlupkova Z, Quatrano RS, Fowler JE (2000) Maize ROP7 GTPase contains a unique, CaaX box-independent plasma membrane targeting signal. Plant J 24:79–90
Kasidimoko NM, Kurosaki F, Lee JB, Hayashi T (2005) Stimulation of calcium signal transduction involves in enhancement of production of scopadulcic acid B by methyl jasmonate in the cultured tissues of Scoparia dulcis. Plant Biotechnol 22:333–337
Kawasaki T, Henmi K, Ono E, Hatakeyama S, Iwano M, Satoh H, Shimamoto K (1999) The small GTP-binding protein Rac is a regulator of cell death in plants. Proc Nat Acad Sci USA 96:10922–10926
Kenmotsu Y, Ogita S, Katoh Y, Yamamura Y, Kurosaki F (2010) Transcriptional activation of putative calmodulin genes Am-cam-1 and Am-cam-2 from Aquilaria microcarpa, in response to external stimuli. Biol Pharm Bull 33:1911–1914
Kenmotsu Y, Ogita S, Katoh Y, Yamamura Y, Takao Y, Tatsuo Y, Fujino H, Kadota S, Kurosaki F (2011) Methyl jasmonate-induced enhancement of expression activity of Am-FaPS-1, a putative farnesyl diphosphate synthase gene from Aquilaria microcarpa. J Nat Med 65:194–197
Kurosaki F, Kaburaki H, Nishi A (1994) Involvement of plasma membrane-located calmodulin in the response decay of cyclic nucleotide-gated cation channel of cultured carrot cells. FEBS Lett 340:193–196
Kurosaki F, Tsurusawa Y, Nishi A (1987) Breakdown of phosphatidylinositol during the elicitation of phytoalexin production in cultured carrot cells. Plant Physiol 85:601–604
Lavy M, Bracha-Drori K, Sternberg H, Yalovsky S (2002) A cell-specific, prenylation-independent mechanism regulates targeting of type II RACs. Plant Cell 14:2431–2450
Li H, Lin Y, Heath RM, Zhu MX, Yang Z (1999) Control of pollen tube tip growth by a Rop GTPase-dependent pathway that leads to the tip-localized calcium influx. Plant Cell 11:1731–1742
Li H, Shen J, Zheng Z, Lin Y, Yang Z (2001) The Rop GTPase switch controls multiple developmental processes in Arabidopsis. Plant Physiol 126:670–684
Lu X, Lin X, Shen Q, Zhang F, Wang Y, Chen Y, Wang T, Wu S, Tang K (2011) Characterization of the jasmonate biosynthetic gene allene oxide cyclase in Artemisia annua L., source of the antimalarial drug artemisinin. Plant Mol Biol Rep 29:489–497
Mitamura T, Shite M, Yamamura Y, Kurosaki F (2009) Cloning and characterization of a gene encoding Rac/Rop-like monomeric guanosine 5′-triphosphate-binding protein from Scoparia dulcis. Biol Pharm Bull 32:1122–1125
Mitamura T, Yamamura Y, Kurosaki F (2011) Modification and translocation of Rac/Rop guanosine 5′-triphosphate-binding proteins of Scoparia dulcis in response to stimulation with methyl jasmonate. Biol Pharm Bull 36:845–849
Mitsuma S, Ishigaki E, Sugiyama R, Asamizu T, Yamada K, Kurosaki F (2004) Activation of phenylpropanoid metabolism in sesame by over-expression of carrot calmodulin gene. Biol Pharm Bull 27:1621–1625
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–479
Ng LT, Chang YS, Kadir AA (1997) A review on agar (gaharu) producing Aquilaria species. J Trop Forest Prod 2:272–285
Okudera Y, Ito M (2009) Production of agarwood fragrant constituents in Aquilaria calli and suspension cultures. Plant Biotechnol 26:307–315
Ono E, Wong HL, Kawasaki T, Hasegawa M, Kodama O, Shimamoto K (2001) Essential role of the small GTPase Rac in disease resistance of rice. Proc Nat Acad Sci USA 98:759–764
Saitoh D, Asakura Y, Kasidimoko NM, Shite M, Sugiyama R, Lee JB, Hayashi T, Kurosaki F (2007) Cloning and expression of calmodulin gene in Scoparia dulcis. Biol Pharm Bull 30:1161–1163
Shite M, Yamamura Y, Kurosaki F (2009) Cloning and transcriptional regulation of Sdrac encoding a Rac/Rop small guanosine 5′-triphosphate-binding protein gene from Scoparia dulcis. Plant Biotechnol 26:403–408
Steinhilb ML, Turner RS, Gaut JR (2001) The protease inhibitor, MG132, blocks maturation of the amyloid precursor protein Swedish mutant preventing cleavage by beta-Secretase. J Biol Chem 276:4476–4484
Ueda J, Imamura L, Tezuka Y, Tran QL, Tsuda M, Kadota S (2006) New sesquiterpene from Vietnamese agarwood and its induction effect on brain-derived neurotrophic factor mRNA expression in vitro. Bioorg Med Chem 14:3571–3574
Wang CT, Shao JM (2012) Characterization of the ZmCK1 gene encoding a calcium-dependent protein kinase responsive to multiple abiotic stresses in maize. Plant Mol Biol Rep. doi:10.1007/s11105-012-0496-5
Zambounis AG, Kalamaki MS, Tani EE, Paplomatas EJ, Tsaftaris AS (2012) Expression analysis of defense-related genes in cotton (Gossypium hirsutum) after Fusarium oxysporum f. sp. vasinfectum infection and following chemical elicitation using a salicylic acid analog and methyl jasmonate. Plant Mol Biol Rep 30:225–234
Zeng ZL, Yang Z (2000) The Rop GTPase: an emerging signaling switch in plants. Plant Mol Biol 44:1–9
Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333
Acknowledgments
This work was supported, in part, by research grants from the Ministry of Education, Culture, Sports, Science & Technology in Japan, and from Cosmetology Research Foundation.
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Kenmotsu, Y., Asano, K., Yamamura, Y. et al. Cloning and Expression of Putative Rac/Rop GTPase Genes, Am-rac1 and Am-rac2, Involved in Methyl Jasmonate-Induced Transcriptional Activation of Farnesyl Diphosphate Synthase in Cell Cultures of Aquilaria microcarpa . Plant Mol Biol Rep 31, 539–546 (2013). https://doi.org/10.1007/s11105-012-0529-0
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DOI: https://doi.org/10.1007/s11105-012-0529-0