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Differential Expression of Rice Lambda Class GST Gene Family Members During Plant Growth, Development, and in Response to Stress Conditions

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Abstract

Glutathione S-transferases (GSTs; EC 2.5.1.18) are members of an isozyme family and catalyze the conjugation of the reduced tripeptide glutathione to a variety of hydrophobic and electrophilic substrates. Though members of different classes of the GST superfamily have been identified and characterized from many plant species including rice, no detailed information is available for the Lambda class gene family in rice. In this study, a genome-wide analysis was carried out to investigate expression patterns of three Lambda class GST members of rice including OsGSTL1, OsGSTL2 and OsGSTL3 in seedlings, at different growth and developmental stages as well as in response to various biotic and abiotic stresses. Expression analysis using microarray datasets and quantitative real-time reverse transcriptase polymerase chain reaction suggests that this gene family express differentially in various tissues, in response to hormones and during different biotic and abiotic stresses including heavy metals, cold, drought and salt stress. Massively Parallel Signature Sequencing (MPSS) analysis also showed differential expression of OsGSTLs during plant growth and development and under different stresses. Out of three members, maximum expression of OsGSTL2 was observed for the MPSS libraries in comparison to other members. We conclude that members of rice Lambda class GST family play an important role in plant growth and development and in combating different biotic and abiotic stresses.

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Acknowledgments

This work was supported by research grants from the Council of Scientific and Industrial Research, New Delhi, as Network Project and Department of Science and Technology, New Delhi. SK acknowledges the Council of Scientific and Industrial Research Govt. of India for Senior Research Fellowship.

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Authors and Affiliations

  1. CSIR-National Botanical Research Institute, Rana Pratap Marg, Lucknow, 226 001, India

    Smita Kumar, Mehar Hasan Asif, Debasis Chakrabarty, Rudra Deo Tripathi & Prabodh Kumar Trivedi

  2. Department of Biochemistry, Faculty of Science, Banaras Hindu University, Varanasi, 221005, India

    Rama Shanker Dubey

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  1. Smita Kumar
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  2. Mehar Hasan Asif
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  3. Debasis Chakrabarty
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Correspondence to Prabodh Kumar Trivedi.

Electronic Supplementary Materials

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Supplementary Table S1

Details of rice microarray experiments from GEO database used in the study (DOC 71 kb)

Supplementary Table S2

List of oligonucleotides used for the analysis (DOC 30 kb)

Supplementary Table S3

MPSS library information used for the OsGSTLs analysis (DOC 56 kb)

Supplementary Table S4

List of all AtGSTs and OsGSTs, In2-1 proteins considered for the phylogenetic analysis (DOC 86 kb)

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Kumar, S., Asif, M.H., Chakrabarty, D. et al. Differential Expression of Rice Lambda Class GST Gene Family Members During Plant Growth, Development, and in Response to Stress Conditions. Plant Mol Biol Rep 31, 569–580 (2013). https://doi.org/10.1007/s11105-012-0524-5

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