Plant Molecular Biology Reporter

, Volume 31, Issue 2, pp 435–442 | Cite as

Overexpression of the GmNAC2 Gene, an NAC Transcription Factor, Reduces Abiotic Stress Tolerance in Tobacco

  • Hangxia Jin
  • Fang Huang
  • Hao Cheng
  • Haina Song
  • Deyue Yu
Original Paper

Abstract

NAC (NAM, ATAF1, 2, and CUC2) proteins play important roles in plant development and stress responses. Glycine max NAC-like gene 2 (GmNAC2) is the first ATAF1-like NAC transcription factor identified in soybean. In this study, GmNAC-2-overexpressing tobacco lines were developed and found to be hypersensitive to drought, high salinity, and cold stress. Under abiotic stress, the transgenic tobacco leaves had higher malondialdehyde (MDA) levels compared with wild-type plants. An elevated MDA level is used frequently as an indicator of reactive oxygen species (ROS) and associated cell membrane degradation or dysfunction. To determine the mechanism of GmNAC2-mediated stress intolerance, leaf transcriptome analyses were performed comparing GmNAC2-overexpressing tobacco with wild-type tobacco. Identified downregulated genes included genes related to ROS scavenging in GmNAC2-overexpressing tobacco. These results indicate that GmNAC2 functions as a negative regulator during abiotic stress, and participates in ROS signaling pathways through modulation of the expression of genes related to ROS-scavenging.

Keywords

GmNAC2 Soybean Abiotic stress Transgenic tobacco Transcriptome 

Notes

Acknowledgments

This work was supported in part by the National Basic Research Program of China (973 Program) (nos. 2010CB125906 and 2009CB118400) and the National Natural Science Foundation of China (nos. 30800692, 31000718, and 31171573).

Supplementary material

11105_2012_514_MOESM1_ESM.xls (35 kb)
Table S1111 genes showing at least 2-fold expression changes in GmNAC2-overexpressing tobacco leaves compared with the wild type (XLS 35 kb)

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Hangxia Jin
    • 1
  • Fang Huang
    • 1
  • Hao Cheng
    • 1
  • Haina Song
    • 1
  • Deyue Yu
    • 1
  1. 1.National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm EnhancementNanjing Agricultural UniversityNanjingChina

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