Identification and Expression Pattern of a ZPR1 Gene in Wild Tomato (Solanum Pennellii)
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The mammalian zinc finger protein ZPR1 is known to be essential in cell cycle progression, but its homolog in plants remains to be identified. Here, we isolated SpZPR1 from the wild tomato species Solanum pennellii LA0716 and found that its protein sequence shares homology with ZPR1 genes from other organisms. SpZPR1 mRNA was found to accumulate in tomato apical meristem in response to ABA (abscisic acid) treatment and heat stress. A transactivation activity assay in yeast revealed that SpZPR1 functions as a transcriptional activator, while the results of our gel electrophoresis mobility shift assay showed that SpZPR1 was able to bind the ABRE (ABA-responsive element) sequence in vitro. Our study is the first to provide evidence that SpZPR1 is involved in the ABA signaling network and plays a potential role in plant cell development and abiotic stress response.
KeywordsAbscisic acid Tomato Zinc finger ZPR1
We thank Dr. Khurram Ziaf for critical reviews of this manuscript. This work was supported by grants from Project 973 (NO. 2009CB119002), the National High Technology R&D Program of China (2012AA100104) and the National Natural Science Foundation of China (30871712).
- Fujita Y, Nakashima K, Yoshida T, Katagiri T, Kidokoro S, Kanamori N, Umezawa T, Fujita M, Maruyama K, Ishiyama K, Kobayashi M, Nakasone S, Yamada K, Ito T, Shinozaki K, Yamaguchi-Shinozaki K (2009) Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis. Plant Cell Physiol 50:2123–2132PubMedCrossRefGoogle Scholar
- Peng S, Zhu Z, Zhao K, Shi J, Yang Y, He M, Wang Y (2012) A novel heat shock transcription factor, VpHsf1, from Chinese wild Vitis pseudoreticulata is involved in biotic and abiotic stresses. Plant Mol Biol Rep: 1–8Google Scholar
- Wu F, Mueller LA, Crouzillat D, Petiard V, Tanksley SD (2006) Combining bioinformatics and phylogenetics to identify large sets of single-copy orthologous genes (COSII) for comparative, evolutionary and systematic studies: a test case in the euasterid plant clade. Genetics 174:1407–1420PubMedCrossRefGoogle Scholar