Plant Molecular Biology Reporter

, Volume 31, Issue 2, pp 352–362 | Cite as

Mapping of a Heat-Stable Gene for Resistance to Southern Root-Knot Nematode in Solanum lycopersicum

  • Yinlei Wang
  • Wencai Yang
  • Wei Zhang
  • Qing Han
  • Miao Feng
  • Huolin Shen
Original Paper

Abstract

Root-knot nematodes (Meloidogyne spp.) can cause severe problems in tomato production in warm climates. To date, Mi-1 is the only gene that has been used widely to develop cultivars for controlling disease caused by nematodes around the world. However, Mi-1 does not provide resistance to the pest when the soil temperature is above 28 °C. Tomato breeding line ZN17 has been reported to possess resistance to Meloidogyne incognita at high temperatures (32 °C). To identify markers linked tightly to resistance, an F2 population was developed by crossing the resistance line ZN17 to susceptible line 09C84. Analysis of F2 individuals by inoculating M. incognita suggested that resistance in ZN17 is conditioned by a single dominant gene temporarily designated as Mi-HT. Linkage analysis suggested that Mi-HT is located on the short arm of chromosome 6. One marker, W737, co-segregated with Mi-HT. Comparisons of map positions of Mi-HT, Mi-1, and Mi-9, as well as marker genotypes in LA2157, Motelle, and ZN17 suggested that Mi-HT might be a homologue of Mi-1 and Mi-9 or a new gene. The results obtained in this study will facilitate fine-mapping and cloning of the gene as well as marker-assisted breeding for heat-stable resistance to southern root-knot nematodes in tomato.

Keywords

Tomato Heat-stable resistance Root-knot nematode Mapping 

Notes

Acknowledgment

The work was supported by Chinese National Key Project (2009ZX08009-064B).

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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Yinlei Wang
    • 1
  • Wencai Yang
    • 1
  • Wei Zhang
    • 1
  • Qing Han
    • 1
  • Miao Feng
    • 1
  • Huolin Shen
    • 1
  1. 1.Department of Vegetable ScienceChina Agricultural UniversityBeijingChina

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