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Characterization of ADP-Glucose Pyrophosphorylase Encoding Genes in Source and Sink Organs of Maize

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Abstract

ADP-glucose pyrophosphorylase catalyzes the first and limiting step in starch biosynthesis. Six cDNA sequences encoding three large subunits and three small subunits of maize AGPase from database were mined and subsequently named: agpl1, agpl2, agpl3, agps1, agps2, and agps3. To elucidate the roles of these isogenes, a comprehensive expression analysis of the gene family was conducted by quantitative real-time RT-PCR. Based on the expression patterns, the six genes can be divided into three groups: (1) steady expressers (agpl1, agps1, and agpl2), which were expressed relatively constantly both in leaf and grain; (2) tissue and development-specific expressers (agpl3 and agps2), which were expressed only in grain at middle and late development phases; (3) tissue-specific expressers (agps3), whose transcripts kept constant during grain filling and were observed only in grain. In order to clarify the effects of sugar and plant hormone on maize AGPase genes expression, a serial of treatments were used. The results showed that AGPase genes significantly differed in response to sugar and hormone inductions. Enormous transcript changes of these genes could be observed in glucose and sucrose treatments. Interestingly, synergistic effect of ABA and sucrose on these genes was observed.

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Abbreviations

DAP:

Day after pollination

AGPase:

ADP-glucose pyrophosphorylase

ADPG:

Adenosine diphosphate glucose

Glc-1-P:

Glucose-1-phosphate

Glc-6-P:

Glucose-6-phosphate

bt1 :

Brittle 1

sh2 :

Shrunken 2

bt2 :

Brittle 2

ABA:

Abscisic acid

BA:

N(6)-benzyladenine

2,4-D:

2,4-Dichlorophenoxyacetic acid

Pi:

Orthophosphate

PGA:

3-phosphoglycerate

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Acknowledgments

This work was supported by National Natural Science Foundation of China (31071354), National High Technology Research and Development Program 863 in China (‘863 program’ Grant No: 2008AA10Z123) and the National Transgenic Major Program(2009ZX08003-022B). The authors are very grateful to Professor Alan Myers and Dr. Tracie Hennen-Bierwagen (Iowa State University) for helpful discussions.

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Authors and Affiliations

  1. Maize Research Institute, Sichuan Agricultural University, Ya’an, 625014, China

    Binquan Huang, Jiang Chen, Mengliang Tian, Yong Gu, Yufeng Hu, Yangping Li, Yinghong Liu & Yubi Huang

  2. College of Life Science, Sichuan Agricultural University, Ya’an, 625014, China

    Junjie Zhang & Hanmei Liu

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  1. Binquan Huang
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  2. Jiang Chen
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Correspondence to Yubi Huang.

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Huang, B., Chen, J., Zhang, J. et al. Characterization of ADP-Glucose Pyrophosphorylase Encoding Genes in Source and Sink Organs of Maize. Plant Mol Biol Rep 29, 563–572 (2011). https://doi.org/10.1007/s11105-010-0262-5

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