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Cloning and Expression Analysis of the Smooth-Edge Seed Gene GSE-1 from Cucurbita pepo L

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Abstract

The nearly isogenic lines of Cucurbita pepo L designated 7-11-1 and 7-11-2, which possess characters of smooth-edged seeds and reductus-edged seeds, respectively, were subjected to complementary DNA (cDNA)-randomly amplified polymorphic DNA (RAPD) technology to assess differential gene transcription in seed coats. Gene fragments showing differential expression were obtained. Full-length mRNA was recovered by rapid amplification of cDNA ends and the corresponding gene designated GSE-1. BLAST analysis in internet database indicated that this is a novel gene of 1,225 bp contains an open reading frame (ORF) of 903 bp and two typical zinc finger domains. Amplification of this gene from two different inbred lines indicated that the 55th nucleotide of the ORF in the 7-11-2 inbred line had mutated from C to T, thereby terminating the ORF prematurely. Spatial and temporal analysis of gene expression by RT-PCR indicates that GSE-1 is a novel gene specifically expressed in seed coats that displays temporal specificity during development.

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References

  • Buchner P, Rochat C, Wuilleme S, Boutin JP (2002) Characterization of a tissue-specific and developmentally regulated beta-1, 3-glucanase gene in pea (Pisum sativum). Plant Mol Biol 49:171–186

    Article  PubMed  CAS  Google Scholar 

  • Diaz I, Vicente-Carbajosa J, Abraham Z, Martinez M, Isabel-La Moneda I, Carbonero P (2002) The GAMYB protein from barley interacts with the DOF transcription factor BPBF and activates endosperm-specific genes during seed development. Plant J 29:453–464

    Article  PubMed  CAS  Google Scholar 

  • Jagadeesh NS, Natchimuthu K, Ayyanar G, Bharathi KK, Rajasekaran RMN (2009) Identification of a novel drought tolerance gene in Gossypium hirsutum L. cv KC3. Commun Biometry Crop Sci 4:9–13

    Google Scholar 

  • Jiang SY, Chen QF, Fang XJ (1999) Identification of differentially expressed genes in photo-period sensitive genic male sterile rice by randomly amplified cDNAs using RAPD primers. Chin Sci Bull 44:348–351

    Article  CAS  Google Scholar 

  • Lin LS, Varner JE (1991) Expression of ascorbic acid oxidase in zucchini squash (Cucurbita pepo L.). Plant Physiol 96:159–165

    Article  PubMed  CAS  Google Scholar 

  • Macknight R, Bancroft I, Page T, Lister C, Schmidt R, Love K, Westphal L, Murphy G, Sherson S, Cobbett C, Dean C (1997) FCA, a gene controlling flowering time in Arabidopsis, encodes a protein containing RNA-binding domains. Cell 89:737–745

    Article  PubMed  CAS  Google Scholar 

  • Manzano S, Gómez P, Garrido D, Jamilena M (2008) Cloning and characterisation of a CTR1-like gene in Cucurbita pepo, Cucurbitaceae 2008. Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae, INRA, Avignon (France), May 21–24th, 575–580

  • Sambrook J, Russell DW (2002) Molecular cloning: a laboratory manual. Beijing http://www.openj-gate.com/Browse/ArticleList.aspx?Journal_id=126765&issue_id=1052293#

  • Siva KP, Sham SK (2008) Identification of differentially expressed molecular markers for breast cancer diagnosis by cDNA-RAPD. Curr Trends Biotechnol Pharm 2:156–169

    Google Scholar 

  • Xie GS, Liu SK, Takano T, You ZB, Zhang DP (2002) Cloning and expression of VB12-independent methionine synthase gene responsive to alkaline stress in rice. Yi Chuan Xue Bao 29:1078–1084

    PubMed  CAS  Google Scholar 

  • Yamagata H, Yonesu K, Hirata A, Aizono Y (2002) TGTCACA motif is a novel cis-regulatory enhancer element involved in fruit-specific expression of the cucumisin gene. J Biol Chem 277:11582–11590

    Article  PubMed  CAS  Google Scholar 

  • Yoshida KT, Naito S, Takeda G (1994) cDNA cloning of regeneration-specific genes in rice by differential screening of randomly amplified cDNAs using RAPD primers. Plant Cell Physiol 35:1003–1009

    PubMed  CAS  Google Scholar 

  • Yu H, Yang SH, Goh CJ (2002) Spatial and temporal expression of the orchid floral homeotic gene DOMADS1 is mediated by its upstream regulatory regions. Plant Mol Biol 49:225–237

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by grants from the National Science and Technology Pillar Program in the 11th 5-year Plan of China (2006BAD04B08, 2007BAD88B03), the National Sparking Plan Program (2008GA630003) and the Special Scientific Research Fund for the Public Service Sector (NYHYZX-07-007). We are grateful for molecular biology technical support provided by the School of Life Sciences in Shanxi University.

Competing Interests

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. College of Horticulture, Shanxi Agricultural University, Taigu, 030801, China

    Shang Guo

  2. Institute of Vegetable Research, Shanxi Academy of Agricultural Science, Taiyuan, 030031, China

    Shang Guo, Ruxia Tian, Zhiwei Feng & Chengfeng Zhao

  3. College of Horticulture, Shanxi Agricultural University, Taiyuan, 030025, China

    Shang Guo

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  1. Shang Guo
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  2. Ruxia Tian
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  3. Zhiwei Feng
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  4. Chengfeng Zhao
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Correspondence to Shang Guo.

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Supplementary Table 1

The RAPD primers sequence information (DOC 89 kb)

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Guo, S., Tian, R., Feng, Z. et al. Cloning and Expression Analysis of the Smooth-Edge Seed Gene GSE-1 from Cucurbita pepo L. Plant Mol Biol Rep 29, 498–504 (2011). https://doi.org/10.1007/s11105-010-0253-6

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