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Phylogenetic Analysis of S-Locus Genes Reveals the Complicated Evolution Relationship of S Haplotypes in Brassica

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Abstract

Self-incompatibility (SI) is reported to play a key role in the evolution of species as it promotes their outcrossing through the recognition and rejection of self-pollen grains. In Brassica, two S-locus genes expressed in the stigma, S-locus glycoprotein (SLG) gene and S-locus receptor kinase (SRK) gene, and one expressed in the pollen, S-locus protein 11 (SP11) gene, were linked as an S haplotype. In order to analyze the evolutionary relationships of S haplotypes in Brassica, a total of 39 SRK, 37 SLG, and 58 SP11 sequences of Brassica oleracea, Brassica rapa and Brassica napus were aligned. Two phylogenetic trees with similar pattern were constructed based on the nucleotide sequences of SRK/SLG and SP11, respectively. Class I and class II alleles were clustered into two distinct groups, and alleles from different species, including all the interspecific pairs of S haplotypes, were closely related to each other. The S-locus genes identified in B. napus were intermingled in phylogenetic trees. All these observations showed that class I and class II S haplotypes diverged ahead of the species differentiation in Brassica. The evolution and the genetic diversity of S haplotypes in Brassica were discussed. Moreover, the relationships between S haplotypes and SI phenotypes in Brassica, especially in B. napus, were also discussed.

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Acknowledgments

This research was financed by funds from the “863” Project (2009AA101105) and supported by the Program for Changjiang Scholar and Innovative Research Team in University (IRT0442).

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Authors and Affiliations

  1. College of Agronomy, Henan Agricultural University, 95 Wenhua Road, 450002, Zhengzhou, China

    Xingguo Zhang & Dongmei Yin

  2. National Key Laboratory of Crop Genetic Improvement, National Center of Rapeseed Improvement in Wuhan, Huazhong Agricultural University, 430070, Wuhan, China

    Chaozhi Ma & Tingdong Fu

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  1. Xingguo Zhang
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  2. Dongmei Yin
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  3. Chaozhi Ma
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  4. Tingdong Fu
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Correspondence to Chaozhi Ma.

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Zhang, X., Yin, D., Ma, C. et al. Phylogenetic Analysis of S-Locus Genes Reveals the Complicated Evolution Relationship of S Haplotypes in Brassica . Plant Mol Biol Rep 29, 481–488 (2011). https://doi.org/10.1007/s11105-010-0251-8

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