Abstract
SAP and MIZ (SIZ) is a small ubiquitin-related modifier (SUMO) E3 ligase that facilitates conjugation of SUMO to protein substrates. Although there have been a number of reports about the functions of SIZ1 in Arabidopsis in the regulation of diverse life processes, no information regarding the role of SIZ in other plants is available yet. In this work, two homologous genes from rice (Oryza sativa) were isolated and designated as OsSIZ1 and OsSIZ2 based on amino acid sequence homology to AtSIZ1 and their phylogenetic relationship. The function in the vegetative growth and reproductive development in rice was investigated using OsSIZ1 mutants containing a T-DNA insertion. The results showed that the mutant Ossiz1 exhibited the significant changes in several growth and developmental parameters, including primary root length, adventitious root number, plant height, leaf and panicle length, flower formation, and seed-setting rate compared with wild type. Taking together these results indicate that OsSIZ1 plays an important role in regulating growth and development in rice.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- SIZ:
-
SAP and MIZ
- SUMO:
-
Small ubiquitin-related modifier
References
Beemster GT, De Vusser K, De Tavernier E, De Bock K, Inze D (2002) Variation in growth rate between Arabidopsis ecotypes is correlated with cell division and A-typecyclin-dependent kinase activity. Plant Physiol 129:854–864
Bochar DA, Savard J, Wang W, Lafleur DW, Moore P, Cote J, Shiekhattar R (2000) A family of chromatin remodeling factors related to Williams syndrome transcription factor. Proc Natl Acad Sci USA 97:1038–1043
Bossis G, Melchior F (2006) SUMO: regulating the regulator. Cell Div 1:13
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH (2007) The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses. Plant Cell 19:2952–2966
Coscoy L, Ganem D (2003) PHD domains and E3 ubiquitin ligases: viruses make the connection trends. Cell Biol 13:7–12
Dittmar GA, Wilkinson CR, Jedrzejewski PT, Finley D (2002) Role of a ubiquitin-like modification in polarized morphogenesis. Science 295:2442–2446
Gill G (2004) SUMO and ubiquitin in the nucleus: different functions, similar mechanisms? Genes Dev 18:2046–2059
Hochstrasser M (2000) Evolution and function of ubiquitin-like protein-conjugation systems. Nat Cell Biol 2:E153–E157
Huang L, Yang S, Zhang S, Liu M, Lai J, Qi Y, Shi S, Wang J, Wang Y, Xie Q, Yang C (2009) The Arabidopsis SUMO E3 ligase AtMMS21, a homologue of NSE2/MMS21, regulates cell proliferation in the root. Plant J 60:666–678
Jia L, Zhang B, Mao C, Li J, Wu Y, Wu P, Wu Z (2008) OsCYT-INV1 for alkaline/neutral invertase is involved in root cell development and reproductivity in rice (Oryza sativa L.). Planta 228:51–59
Jin JB, Jin YH, Lee J, Miura K, Yoo CY, Kim WY et al (2008) The SUMO E3 ligase, AtSIZ1, regulates flowering by controlling a salicylic acid-mediated floral promotion pathway and through effects on FLC chromatin structure. Plant J 53:530–540
Kerscher O, Felberbaum R, Hochstrasser M (2006) Modification of proteins by ubiquitin and ubiquitin-like proteins. Annu Rev Cell Dev Biol 22:159–180
Lee J, Nam J, Park HC, Na G, Miura K, Jin JB et al (2007) Salicylic acid-mediated innate immunity in Arabidopsis is regulated by SIZ1 SUMO E3 ligase. Plant J 49:79–90
Li BZ, Xin WJ, Sun SB, Xu GH (2006) Physiological and molecular responses of nitrogen-starved rice plants to re-supply of different nitrogen sources. Plant Soil 287:145–159
Miura K, Jin JB, Hasegawa PM (2007a) Sumoylation, a post-translational regulatory process in plants. Curr Opin Plant Biol 10:495–502
Miura K, Jin JB, Lee J, Yoo CY, Stirm V, Miura T, Ashworth EN, Bressan RA, Yun DJ, Hasegawa PM (2007b) SIZ1-mediated sumoylation of ICE1 controls CBF3/DREB1A expression and freezing tolerance in Arabidopsis. Plant Cell 19:1403–1414
Miura K, Lee J, Jin JB, Yoo CY, Miura T, Hasegawa PM (2009) Sumoylation of ABI5 by the Arabidopsis SUMO E3 ligase SIZ1 negatively regulates abscisic acid signaling. Proc Natl Acad Sci USA 106:5418–5423
Miura K, Lee J, Miura T, Hasegawa PM (2010) SIZ1 controls cell growth and plant development in Arabidopsis through salicylic acid. Plant Cell Physiol 51:103–113
Miura K, Rus A, Sharkhuu A, Yokoi S, Karthikeyan AS, Raghothama KG et al (2005) The Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses. Proc Natl Acad Sci USA 102:7760–7765
Mouchel CF, Briggs GC, Hardtke CS (2004) Natural genetic variation in Arabidopsis identifies BREVIS RADIX, a novel regulator of cell proliferation and elongation in the root. Genes Dev 18:700–714
Reeves PH, Murtas G, Dash S, Coupl G (2002) Early in short days 4, a mutation in Arabidopsis that causes early flowering and reduces the mRNA abundance of the floral repressor FLC. Development 129:5349–5361
Yoo CY, Miura K, Jin JB, Lee J, Park HC, Salt DE, Yun DJ, Bressan RA, Hasegawa PM (2006) SIZ1 small ubiquitin-like modifier E3 ligase facilitates basal thermotolerance in Arabidopsis independent of salicylic acid. Plant Physiol 142:1548–1558
Zhu Y, Dong A, Meyer D, Pichon O, Renou JP, Cao K, Shen WH (2006) Arabidopsis NRP1 and NRP2 encode histone chaperones and are required formaintaining postembryonic root growth. Plant Cell 18:2879–2892
Acknowledgements
This work was supported by a grant from the Ph.D. Program Foundation of the Ministry of Education of China (20090097110038) and the National Basic Research and Development Program of China (2005CB120903).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, H., Makeen, K., Yan, Y. et al. OsSIZ1 Regulates the Vegetative Growth and Reproductive Development in Rice. Plant Mol Biol Rep 29, 411–417 (2011). https://doi.org/10.1007/s11105-010-0232-y
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11105-010-0232-y