Skip to main content
SpringerLink
Log in

Cloning and Functional Characterization of a Novel Glutathione S-Transferase Gene from Limonium bicolor

  • Published:
Plant Molecular Biology Reporter Aims and scope Submit manuscript

Abstract

Plant glutathione S-transferases (GSTs) are involved in protecting plants against both diverse biotic and abiotic stresses. In the present study, a novel GST gene (LbGST1) was cloned from Limonium bicolor (Bunge) Kuntze (Plumbaginaceae). To characterize its function in salt tolerance, tobacco lines transformed with LbGST1 were generated. Compared with wild-type (WT) tobacco, transgenic plants overexpressing LbGST1 exhibited both GST and glutathione peroxidase activities. Moreover, superoxide dismutase, peroxidase (POD), and catalase activities in transgenic plants were significantly higher than those in WT plants, particularly when grown under conditions of salt stress. Similarly, levels of proline in transgenic plants were also higher than those in WT plants grown under NaCl stress conditions. Whereas, Malondialdehyde contents in transgenic plants were lower than those in WT plants under NaCl conditions. Furthermore, Na+ content in transgenic plants was lower than that in WT plants under these stress conditions. Subcellular localization analysis revealed that the LbGST1 protein was localized in the nucleus. These results suggested that overexpression of LbGST1 gene can affect many physiological processes associated with plant salt tolerance. Therefore, we hypothesize that LbGST1 gene can mediate many physiological pathways that enhance stress resistance in plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

Abbreviations

bp:

base pair

CAT:

catalase

GPX:

glutathione peroxidase

GST:

gluthathione S-transferase

GFP:

green fluorescent protein

MDA:

malondialdehyde

RLG:

relative rate of length growth

PCR:

polymerase chain reaction

POD:

peroxidase

SOD:

superoxide dismutase

WT:

wild type

References

  • Bailly C (2004) Active oxygen species and antioxidants in seed biology. Seed Sci Res 14:93–107

    Article  CAS  Google Scholar 

  • Dixon DP, Lapthorn A, Edwards R (2002a) Plant glutathione transferases. Genome Biol 3:3004.1–3004.10

    Article  Google Scholar 

  • Dixon DP, Davis BG, Edwards R (2002b) Functional divergence in the glutathione transferase superfamily in plants. J Biol Chem 277:30859–30869. doi:10.1074/jbc.M202919200

    Article  CAS  PubMed  Google Scholar 

  • Edwards R, Dixon DP, Walbot V (2000) Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health. Trends Plant Sci 5:193–198. doi:10.1016/S1360-1385(00)01601-0

    Article  CAS  PubMed  Google Scholar 

  • Edwards R, Dixon DP (2005) Plant glutathione transferases. Methods Enzymol 401:169–186

    Google Scholar 

  • Frova C (2003) The plant glutathione transferase gene family: Genomic structure, functions, expression and evolution. Physiol Plant 119:469–479

    Article  CAS  Google Scholar 

  • Kampranis SC, Damianova R, Atallah M, Toby G, Kondi G, Tsichlis PN, Makris AM (2000) A novel plant glutathione S-transferase/peroxidase suppresses Bax lethality in yeast. J Biol Chem 275:29207–29216. doi:10.1074/jbc.M002359200

    Article  CAS  PubMed  Google Scholar 

  • Kim YH, Lim S, Han SH, Lee JC, Song WK, Bang JW, Kwon SY, Lee HS, Kwak SS (2007) Differential expression of 10 sweetpotato peroxidases in response to sulfur dioxide, ozone, and ultraviolet radiation. Plant Physiol Biochem 45:908–914. doi:10.1016/j.plaphy.2007.07.019

    Article  CAS  PubMed  Google Scholar 

  • Lim JD, Hahn SJ, Yu CY, Chung I (2005) Expression of the glutathione S-transferase gene (NT107) in transgenic Dianthus superbus. Plant Cell Tiss Org 80:277–286. doi:10.1007/s11240-004-1032-6

    Article  CAS  Google Scholar 

  • Kishor P, Hong Z, Miao GH, Hu C, Verma D (1995) Overexpression of [delta]-pyrroline-5-carboxylate synthetase increases proline production and confers osmotolerance in transgenic plants. Plant Physiol 108:1387–1394

    CAS  PubMed  Google Scholar 

  • Liu XF, Li JY (2002) Characterization of an ultra-violet inducible gene that encodes glutathione S-transferase in Arabidopsis thaliana. Acta Genetic Sinica 29:458–460

    CAS  Google Scholar 

  • Mylona PV, Polidoros AN, Scandalios JG (2007) Antioxidant gene responses to ROS-generating xenobiotics in developing and germinated scutella of maize. J Exp Bot 58:1301–1312. doi:10.1093/jxb/erl292

    Article  CAS  PubMed  Google Scholar 

  • Obata T, Kitamoto HK, Nakamura A, Fukuda A, Tanaka Y (2007) Rice Shaker Potassium Channel OsKAT1 Confers tolerance to salinity stress on yeast and rice cells. Plant Physiol 144:1978–1985. doi:10.1104/pp.107.101154

    Article  CAS  PubMed  Google Scholar 

  • Roxas VP, Smith RK Jr, Allen ER, Allen RD (1997) Overexpression of glutathione S-transferase/glutathione peroxidase enhances the growth of transgenic tobacco seedlings during stress. Nat Biotechnol 15:988–991. doi:10.1038/nbt1097-988

    Article  CAS  PubMed  Google Scholar 

  • Roxas VP, Lodhi SA, Garrett DK, Mahan JR, Allen RD (2000) Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase. Plant Cell Physiol 41:1229–1234

    Article  CAS  PubMed  Google Scholar 

  • Silva-Ortega CO, Ochoa-Alfaro AE, Reyes-Agüero JA, Aguado-Santacruz GA, Jiménez-Bremont JF (2007) Salt stress increases the expression of p5cs gene and induces proline accumulation in cactus pear. Plant Physiol Biochem 46:82–92. doi:10.1016/j.plaphy.2007.10.011

    Article  PubMed  Google Scholar 

  • Sunkar R, Bartels D, Kirch HH (2003) Overexpression of a stress inducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance. Plant J 35:452–464. doi:10.1046/j.1365-313X.2003.01819.x

    Article  CAS  PubMed  Google Scholar 

  • Takesawa T, Ito M, Kanzaki H, Kameya N, Nakamura I (2002) Over-expression of glutathione S-transferase in transgenic rice enhances germination and growth at low temperature. Mol Breed 9:93–101

    Article  CAS  Google Scholar 

  • Tester M, Davenport R (2003) Na+ tolerance and Na+ transport in higher plants. Ann Bot 91:503–527

    Article  CAS  PubMed  Google Scholar 

  • Verdoy D, Coba De La Peña T, Redondo FJ, Lucas MM, Pueyo JJ (2006) Transgenic Medicago truncatula plants that accumulate proline display nitrogen-fixing activity with enhanced tolerance to osmotic stress. Plant Cell Environ 29:1913–1923. doi:10.1111/j.1365-3040.2006.01567.x

    Article  CAS  PubMed  Google Scholar 

  • Wang Y, Ma H, Liu G, Zhang D, Ban Q, Zhang G, Xu C, Yang C (2008) Generation and analysis of expressed sequence tags from a NaHCO3-treated Limonium bicolor cDNA library. Plant Physiol Biochem 46:977–986. doi:10.1016/j.plaphy.2008.06.001

    Article  CAS  PubMed  Google Scholar 

  • Yu T, Li YS, Chen XF, Hu J, Chang X, Zhu YG (2003) Transgenic tobacco plants overexpressing cotton glutathione S-transferase (GST) show enhanced resistance to methyl viologen. J Plant Physiol 160:1305–1311. doi:10.1078/0176-1617-01205

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This work has been supported by Program for New Century Excellent Talents in University (NCET-08-0751), Foundation for the Author of National Excellent Doctoral Dissertation of China (200973), and Science foundation of Northeast Forestry University (09040).

Author information

Authors and Affiliations

  1. Key Laboratory of Forest Tree Genetic improvement and Biotechnology, Northeast Forestry University, Ministry of Education, Harbin, People’s Republic of China

    Guiping Diao, Yucheng Wang, Chao Wang & Chuanping Yang

  2. School of Forestry, Northeast Forestry University, 26 Hexing Road, Harbin, 150040, People’s Republic of China

    Guiping Diao, Yucheng Wang, Chao Wang & Chuanping Yang

Authors
  1. Guiping Diao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yucheng Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chao Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chuanping Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chuanping Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Diao, G., Wang, Y., Wang, C. et al. Cloning and Functional Characterization of a Novel Glutathione S-Transferase Gene from Limonium bicolor . Plant Mol Biol Rep 29, 77–87 (2011). https://doi.org/10.1007/s11105-010-0212-2

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11105-010-0212-2

Keywords

Search

Navigation